| Loss of several cell functions including okadaic acid-induced apoptosis after multiple passages in FRTL-5 cells. | |
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MedLine Citation:
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PMID: 10411308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In FRTL-5 cells, cultured over a period of more than 3 years, different properties of the cells have been observed to undergo spontaneous changes in the course of aging, i.e. after an increase in the number of passages. This consists mainly in alterations in their morphological phenotype and in some of their functional properties. The morphology of the cells displayed a progressive disruption of the monolayer organization with a loss of cell-cell contacts and a marked rounding-up of the cells. The uptake of iodide was not modified nor was the expression of thyroglobulin (Tg) mRNA as determined at various time intervals in the course of the cells culturing. Estimation of the proliferation by counting the frequency of [3H]thymidine labeled nuclei revealed an age-related decline in the sensitivity to TSH mitogenic action associated with a reciprocal increase in the insulin synergistic effect. Aged cells (+/- 40 passages) lost their apoptosis sensitivity to the phosphatase inhibitor, okadaic acid (OA) but not to cycloheximide (CHX) and/or actinomycin D (act. D) exposure. Altogether these observations favor the existence of a shift towards transformed properties with only partial loss of differentiated functions. |
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Authors:
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J Golstein; T Kimura; F Miot; J E Dumont |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 150 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-09-03 Completed Date: 1999-09-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: IRELAND |
Other Details:
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Languages: eng Pagination: 141-9 Citation Subset: IM |
Affiliation:
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Institute of Interdisciplinary Research, Free University of Brussels, Belgium. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects, physiology* Cell Aging / physiology* Cell Division / drug effects, physiology Cell Line Enzyme Inhibitors / pharmacology* Okadaic Acid / pharmacology* Rats Thyroid Gland / pathology, physiology* Thyrotropin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 78111-17-8/Okadaic Acid; 9002-71-5/Thyrotropin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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