| Loss of seeded endothelial cells in vivo. A study of Dacron grafts under different flow conditions. | |
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MedLine Citation:
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PMID: 7828745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To assess, in vivo, the loss of endothelial cells seeded in a Dacron graft at low, restricted arterial flow and at normal unrestricted flow. DESIGN AND SETTING: Laboratory animal study. MATERIALS: Indium-111-oxine labelled endothelial cells were seeded in externally supported, preclotted Dacron grafts, inserted as interposition grafts in the carotid arteries of nine sheep. Activity (radioactivity) was measured, flow established, on one side unrestricted 120-180 ml/minute, and on the other restricted to 50 ml/minute by a distal clamp. OUTCOME MEASURES: The reduction in activity over the grafts was measured for 2 hours. RESULTS: There was an immediate loss of activity to around 80% and thereafter a lower rate of cell loss to around 50%. No difference could be demonstrated in the loss from low flow compared to high flow grafts. All grafts were patent. There was no difference in thrombus weights. CONCLUSION: No difference could be demonstrated in seeded endothelial cell loss from a Dacron graft in vivo at low flow (50 ml/minute) compared with unrestricted flow (120-180 ml/minute). |
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Authors:
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N Jensen; B Linblad; S Leide; D Bergqvist |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of vascular surgery Volume: 8 ISSN: 0950-821X ISO Abbreviation: Eur J Vasc Surg Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1995-02-17 Completed Date: 1995-02-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709440 Medline TA: Eur J Vasc Surg Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 690-3 Citation Subset: IM |
Affiliation:
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Department of Surgery, Lund University, Malmö General Hospital, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Flow Velocity Blood Vessel Prosthesis* Carotid Arteries / surgery Cell Adhesion Cells, Cultured Endothelium, Vascular / cytology* Female Hemorheology Indium Radioisotopes / diagnostic use Organometallic Compounds / diagnostic use Oxyquinoline / analogs & derivatives, diagnostic use Polyethylene Terephthalates* Pulsatile Flow Sheep Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Indium Radioisotopes; 0/Organometallic Compounds; 0/Polyethylene Terephthalates; 14514-42-2/indium oxine; 148-24-3/Oxyquinoline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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