Document Detail

Loss of restriction site DdeI, used for avian molecular sexing, in Oreophasis derbianus.
MedLine Citation:
PMID:  12464068     Owner:  NLM     Status:  MEDLINE    
Molecular sexing is a rapid and safe procedure for bird sex determination. Two universal methods based on the amplification of a chromo-helicase-DNA-Binding 1 (CHD) gene region, located in both sexual chromosomes (Z and W), have been established. We found that molecular sexing of Oreophasis derbianus failed by using these two procedures. One of them is based on a restriction site located in CHD1W gene but absent in CHD1Z. The DdeI restriction site, used successfully to determine gender in several bird species, was found to be lost because of nucleotide change in O. derbianus. This change created a new restriction site, NlaIII, that was successfully applied for sexing this endangered bird.
L G Bermúdez-Humarán; P Chávez-Zamarripa; A Guzmán-Velasco; C H Leal-Garza; R Montes de Oca-Luna
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  37     ISSN:  0936-6768     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-04     Completed Date:  2003-03-03     Revised Date:  2009-10-07    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  321-3     Citation Subset:  IM    
Laboratorio de Inmunología y Virología, Facultad de Ciencias; Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza N.L. México.
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MeSH Terms
Birds / genetics*
DNA Primers
DNA-Binding Proteins / genetics*
Evolution, Molecular
Polymerase Chain Reaction / methods,  veterinary
Polymorphism, Restriction Fragment Length
Restriction Mapping
Sex Chromosomes / genetics
Sex Determination (Analysis) / methods,  veterinary*
Reg. No./Substance:
0/DNA Primers; 0/DNA-Binding Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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