Document Detail


Loss of a putative novel growth inhibitory apoptotic 14 kD polypeptide during progression of rat liver carcinogenesis.
MedLine Citation:
PMID:  9754056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Carcinogenesis is a multistep process involving different stages. However, the biological and biochemical factors responsible for the stepwise transition of cells from one stage to the other remains as important enigmas even today. We have recently isolated a putative novel growth inhibitory apoptotic 14 kD polypeptide from normal rat liver. In order to understand the possible functional relationship between 14 kD polypeptide and liver carcinogenesis, the sequential expression of this polypeptide as a function of tumor progression was studied in the rat liver using diethylnitrosamine (DEN) as a carcinogen. Immunoperoxidase and immunoblotting experiments using polyclonal rabbit antisera revealed a gradual reduction in the levels of this polypeptide with tumor progression. No reduction in the levels of this polypeptide was observed in regenerating rat liver after partial hepatectomy. The findings suggest that the loss or reduction of 14 kD polypeptide is linked selectively to abnormal cell proliferation and appears to be a biologically relevant risk factor for the progression of hepatocarcinogenesis in rats.
Authors:
S S Dadke; S C Krishnamurthy; K V Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Indian journal of experimental biology     Volume:  36     ISSN:  0019-5189     ISO Abbreviation:  Indian J. Exp. Biol.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-10-22     Completed Date:  1998-10-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0233411     Medline TA:  Indian J Exp Biol     Country:  INDIA    
Other Details:
Languages:  eng     Pagination:  245-52     Citation Subset:  IM    
Affiliation:
Cellular Carcinogenesis Laboratory, Tata Memorial Hospital, Tata Memorial Centre, Mumbai, India. cri3@soochak.ncst.ernet.in
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / physiology*
Carcinogens / toxicity
Diethylnitrosamine / toxicity
Immunohistochemistry
Liver / physiology*
Liver Neoplasms, Experimental / chemically induced,  physiopathology*
Male
Peptides / isolation & purification,  physiology*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Carcinogens; 0/Peptides; 55-18-5/Diethylnitrosamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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