| Loss of proteins from digitonin-permeabilized adrenal chromaffin cells essential for exocytosis. | |
| | |
MedLine Citation:
|
PMID: 3680269 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cultured chromaffin cells can be permeabilized with digitonin; the cell interior is then accessible to the cytoplasm, and addition of calcium provokes release of catecholamines. Increasing the incubation time between the permeabilization step and calcium-induced stimulation resulted in a progressive inhibition of secretion reaching 60% after 20 min. Cytosoluble proteins which leak from detergent-permeabilized cells were collected, dialyzed, and concentrated. When these proteins were added back to permeabilized cells which were unable to secrete, catecholamine release was fully restored, suggesting that certain proteins necessary for exocytosis had been dialyzed from these cells. One of the released proteins was characterized as calmodulin. However, addition of calmodulin alone was ineffective in maintaining or restoring secretory activity in digitonin-permeabilized cells, excluding calmodulin as the sole factor responsible for the loss of release. Protein kinase C was also identified as one of the leaked proteins. This enzyme is known to be retained in cells in the presence of 12-O-tetradecanoylphorbol 13-acetate (TPA). However, under TPA-dependent conditions, there was also a loss of secretory activity. The present paper shows that among the proteins leaked from digitonin-permeabilized cells, there are specific proteins crucial to the exocytotic mechanism. |
| | |
Authors:
|
T Sarafian; D Aunis; M F Bader |
Related Documents
:
|
1898329 - Phorbol-ester-mediated expression of the collagen type i pro-alpha 2 gene in mouse 3t3-... 9022759 - Induction of cyclooxygenase-1 in a human megakaryoblastic cell line (cmk) differentiate... 6261139 - Similarity of teleocidin b and phorbol ester tumour promoters in effects on membrane re... 11748689 - Effects of mitogens on synthesis and distribution of heparan and chondroitin sulphates ... 7957639 - Distinct induction of c-fos mrna in ng108-15 cells transfected with muscarinic m1 and m... 7693089 - In vitro investigation on proliferative cystic keratinizing squamous lesions in the rat... 9827879 - Investigations on the nephrotoxicity and hepatotoxicity of trivalent and hexavalent chr... 12819379 - Histochemistry of six lectins in the tissues of the flat fish paralichthys olivaceus. 19903469 - Xiap-mediated protection of h460 lung cancer cells against cisplatin. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 262 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1987 Dec |
Date Detail:
|
Created Date: 1988-01-12 Completed Date: 1988-01-12 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 16671-6 Citation Subset: IM |
Affiliation:
|
Groupe de Neurobiologie Structurale et Fonctionnelle, Unité Institut National de la Santé et de la Recherche Médicale U-44, Strasbourg, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adrenal Medulla
/
cytology* Animals Calcium / pharmacology Calmodulin / pharmacology Cattle Chromaffin Granules / drug effects, metabolism* Chromaffin System / metabolism* Digitonin / pharmacology* Exocytosis* Molecular Weight Norepinephrine / metabolism Permeability Proteins / metabolism* Tetradecanoylphorbol Acetate / pharmacology Type C Phospholipases / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Calmodulin; 0/Proteins; 11024-24-1/Digitonin; 16561-29-8/Tetradecanoylphorbol Acetate; 51-41-2/Norepinephrine; 7440-70-2/Calcium; EC 3.1.4.-/Type C Phospholipases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and some characteristics of an adhesive factor of brain that enhances neurite outgrowth in...
Next Document: Purification and characterization of an Mr 87,000 protein kinase C substrate from rat brain.