| Loss of peroxisomes causes oxygen insensitivity of the histochemical assay of glucose-6-phosphate dehydrogenase activity to detect cancer cells. | |
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MedLine Citation:
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PMID: 17101723 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxygen insensitivity of carcinoma cells and oxygen sensitivity of non-cancer cells in the histochemical assay of glucose-6-phosphate dehydrogenase (G6PD) enables detection of carcinoma cells in unfixed cell smears or cryostat sections of biopsies. The metabolic background of oxygen insensitivity is still not understood completely. In the present study, rat hepatocytes, rat hepatoma cells (FTO-2B), and human colon carcinoma cells (HT29) were used to elucidate these backgrounds. The residual activity in oxygen was 0%, 55%, and 80% in hepatocytes, hepatoma cells, and colon carcinoma cells, respectively. N-ethylmaleimide (NEM), a blocker of SH-groups, did not affect G6PD activity in both carcinoma cell types but reduced G6PD activity in hepatocytes by 40%. Ultrastructural localization of G6PD activity was exclusively in the cytoplasm of carcinoma cells, but in hepatocytes both in cytoplasm and peroxisomes. NEM abolished peroxisomal G6PD activity only. Histochemical assay of catalase activity demonstrated absence of peroxisomes in both carcinoma cell lines. It is concluded that absence of SH-sensitive G6PD activity in peroxisomes in cancer cells is responsible for the oxygen-insensitivity phenomenon. |
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Authors:
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Wilma M Frederiks; Heleen Vreeling-Sindelárová; Cornelis J F Van Noorden |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2006-11-13 |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 55 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-01-22 Completed Date: 2007-02-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: United States |
Other Details:
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Languages: eng Pagination: 175-81 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Histology, Academic Medical Center, Meibergdreef 15 1105 AZ, Amsterdam, The Netherlands. w.m.frederiks@amc.uva.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Catalase / metabolism Cell Line, Tumor Colonic Neoplasms Ethylmaleimide / pharmacology Glucosephosphate Dehydrogenase / metabolism* Hepatocytes / enzymology Histocytochemistry Humans Liver Neoplasms Male Oxygen / metabolism* Peroxisomes / enzymology*, ultrastructure Rats Rats, Wistar Sulfhydryl Reagents / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Sulfhydryl Reagents; 128-53-0/Ethylmaleimide; 7782-44-7/Oxygen; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.11.1.6/Catalase |
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