| Loss of liver fatty acid binding protein significantly alters hepatocyte plasma membrane microdomains. | |
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MedLine Citation:
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PMID: 22223861 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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While lipid-rich microdomains of hepatocyte plasma membranes serve as the major scaffolding regions for cholesterol transport proteins important in cholesterol disposition, little is known regarding intracellular factors regulating cholesterol distribution therein. Based on its ability to bind cholesterol and alter hepatic cholesterol accumulation, the cytosolic liver type fatty acid binding protein (L-FABP) was hypothesized as a candidate protein regulating these microdomains. Compared to wild-type hepatocyte plasma membranes, L-FABP gene ablation significantly increased the proportion of cholesterol-rich microdomains. Lack of L-FABP selectively increased cholesterol, phospholipid (esp. phosphatidylcholine), and branched-chain fatty acid accumulation in the cholesterol-rich microdomains. These cholesterol-rich microdomains are important due to enrichment therein of significant amounts of key transport proteins involved in uptake of: i) cholesterol (SR-B1, ABCA-1, P-gp, SCP-2); ii) fatty acid (FATP); and iii) glucose (GLUT2, GLUT1, insulin receptor). L-FABP gene ablation enhanced the concentration of SCP-2, SR-B1, FATP4, and GLUT1 in the cholesterol-poor microdomains, with functional implications in HDL-mediated uptake and efflux of cholesterol. Thus L-FABP gene ablation significantly impacted the proportion of cholesterol-rich versus poor microdomains in the hepatocyte plasma membrane and altered the distribution of lipids and proteins involved in cholesterol uptake therein. |
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Authors:
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Avery L McIntosh; Barbara P Atshaves; Stephen M Storey; Kerstin K Landrock; Danilo Landrock; Greg G Martin; Ann B Kier; Friedhelm Schroeder |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-5 |
Journal Detail:
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Title: Journal of lipid research Volume: - ISSN: 0022-2275 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Texas A&M University; |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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