Document Detail


Loss of interneuron LTD and attenuated pyramidal cell LTP in Trpv1 and Trpv3 KO mice.
MedLine Citation:
PMID:  23536486     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
TRPV (transient receptor potential, vanilloid) channels are a family of nonselective cation channels that are activated by a wide variety of chemical and physical stimuli. TRPV1 channels are highly expressed in sensory neurons in the peripheral nervous system. However, a number of studies have also reported TRPV channels in the brain, though their functions are less well understood. In the hippocampus, the TRPV1 channel is a novel mediator of long-term depression (LTD) at excitatory synapses on interneurons. Here we tested the role of other TRPV channels in hippocampal synaptic plasticity, using hippocampal slices from Trpv1, Trpv3 and Trpv4 knockout (KO) mice. LTD at excitatory synapses on s. radiatum hippocampal interneurons was attenuated in slices from Trpv3 KO mice (as well as in Trpv1 KO mice as previously reported), but not in slices from Trpv4 KO mice. A previous study found that in hippocampal area CA1, slices from Trpv1 KO mice have reduced tetanus-induced long-term potentiation (LTP) following high-frequency stimulation; here we confirmed this and found a similar reduction in Trpv3 KO mice. We hypothesized that the loss of LTD at the excitatory synapses on local inhibitory interneurons caused the attenuated LTP in the mutants. Consistent with this idea, blocking GABAergic inhibition rescued LTP in slices from Trpv1 KO and Trpv3 KO mice. Our findings suggest a novel role for TRPV3 channels in synaptic plasticity and provide a possible mechanism by which TRPV1 and TRPV3 channels modulate hippocampal output.
Authors:
Travis E Brown; Anda M Chirila; Benjamin R Schrank; Julie A Kauer
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural     Date:  2013-06-03
Journal Detail:
Title:  Hippocampus     Volume:  23     ISSN:  1098-1063     ISO Abbreviation:  Hippocampus     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-08-02     Completed Date:  2014-03-05     Revised Date:  2014-08-04    
Medline Journal Info:
Nlm Unique ID:  9108167     Medline TA:  Hippocampus     Country:  United States    
Other Details:
Languages:  eng     Pagination:  662-71     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Biophysics
Electric Stimulation
GABA Antagonists / pharmacology
Hippocampus / cytology*
Interneurons / drug effects,  physiology*
Long-Term Potentiation / drug effects,  genetics*
Long-Term Synaptic Depression / drug effects,  genetics*
Mice
Mice, Inbred C57BL
Mice, Knockout
Patch-Clamp Techniques
Picrotoxin / pharmacology
Pyramidal Cells / drug effects,  physiology*
Synapses / genetics
TRPV Cation Channels / deficiency*
Grant Support
ID/Acronym/Agency:
F32 NS074612/NS/NINDS NIH HHS; NS065251/NS/NINDS NIH HHS; NS074612/NS/NINDS NIH HHS; R01 DA011289/DA/NIDA NIH HHS; R01 NS065251/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/GABA Antagonists; 0/TRPV Cation Channels; 0/TRPV1 protein, mouse; 0/Trpv3 protein, mouse; 0/Trpv4 protein, mouse; 124-87-8/Picrotoxin
Comments/Corrections

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