Document Detail


Loss of halophytism by interference with SOS1 expression.
MedLine Citation:
PMID:  19571313     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The contribution of SOS1 (for Salt Overly Sensitive 1), encoding a sodium/proton antiporter, to plant salinity tolerance was analyzed in wild-type and RNA interference (RNAi) lines of the halophytic Arabidopsis (Arabidopsis thaliana)-relative Thellungiella salsuginea. Under all conditions, SOS1 mRNA abundance was higher in Thellungiella than in Arabidopsis. Ectopic expression of the Thellungiella homolog ThSOS1 suppressed the salt-sensitive phenotype of a Saccharomyces cerevisiae strain lacking sodium ion (Na(+)) efflux transporters and increased salt tolerance of wild-type Arabidopsis. thsos1-RNAi lines of Thellungiella were highly salt sensitive. A representative line, thsos1-4, showed faster Na(+) accumulation, more severe water loss in shoots under salt stress, and slower removal of Na(+) from the root after removal of stress compared with the wild type. thsos1-4 showed drastically higher sodium-specific fluorescence visualized by CoroNa-Green, a sodium-specific fluorophore, than the wild type, inhibition of endocytosis in root tip cells, and cell death in the adjacent elongation zone. After prolonged stress, Na(+) accumulated inside the pericycle in thsos1-4, while sodium was confined in vacuoles of epidermis and cortex cells in the wild type. RNAi-based interference of SOS1 caused cell death in the root elongation zone, accompanied by fragmentation of vacuoles, inhibition of endocytosis, and apoplastic sodium influx into the stele and hence the shoot. Reduction in SOS1 expression changed Thellungiella that normally can grow in seawater-strength sodium chloride solutions into a plant as sensitive to Na(+) as Arabidopsis.
Authors:
Dong-Ha Oh; Eduardo Leidi; Quan Zhang; Sung-Min Hwang; Youzhi Li; Francisco J Quintero; Xingyu Jiang; Matilde Paino D'Urzo; Sang Yeol Lee; Yanxiu Zhao; Jeong Dong Bahk; Ray A Bressan; Dae-Jin Yun; José M Pardo; Hans J Bohnert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-07-01
Journal Detail:
Title:  Plant physiology     Volume:  151     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-03     Completed Date:  2009-11-23     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  210-22     Citation Subset:  IM    
Affiliation:
Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  metabolism*
Brassicaceae / genetics,  metabolism*
Cell Death / drug effects
Down-Regulation
Gene Expression Regulation, Plant / physiology
Plant Roots / cytology,  metabolism
Plant Shoots / metabolism
RNA Interference
Saccharomyces cerevisiae / genetics,  metabolism
Salt-Tolerant Plants / genetics*,  physiology*
Sodium / metabolism
Sodium Chloride / pharmacology
Sodium-Hydrogen Antiporter / genetics,  metabolism*
Stress, Physiological
Chemical
Reg. No./Substance:
0/SOS1 protein, Arabidopsis; 0/Sodium-Hydrogen Antiporter; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride
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