| Loss of gap junctional intercellular communication in rat lung epithelial cells exposed to carbon or silica-based nanoparticles. | |
| | |
MedLine Citation:
|
PMID: 20868226 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The aim of this study was to investigate whether fine and ultrafine carbon black (fC and ufC), and fine and ultrafine silica (fS, ufS) particles affect gap junctional intercellular communication (GJIC) in rat lung epithelial cells. Exposure of cells to subcytotoxic doses of ufC, fS and ufS resulted in a 63%, 59% and 77% reduction of GJIC, respectively, as determined in a dye transfer assay. In contrast to ufC, fC did not significantly alter GJIC. Changes in subcellular localization of the major gap junction protein in RLE cells, connexin-43 (Cx43), and of β-catenin were observed in cells exposed to ufC, fS or ufS. The loss of GJIC was counteracted by N-acetyl cysteine and was largely prevented by specific inhibitors of epidermal growth factor receptor-dependent signaling, pointing to the crucial role of two known major mediators of nanoparticle action, namely reactive oxygen species and membrane-receptor signaling, in particle-induced modulation of GJIC. |
| | |
Authors:
|
Niloofar Ale-Agha; Catrin Albrecht; Lars-Oliver Klotz |
Related Documents
:
|
7685036 - Epithelial cells retain junctions during mitosis. 18381896 - Control of cell fate by the formation of an architecturally complex bacterial community. 8274766 - Culture and transplantation of the mammalian circadian pacemaker. 1388246 - Cell-to-cell spread of calcium signals mediated by atp receptors in mast cells. 15223676 - Effects of fasting and refeeding on jejunal morphology and cellular activity in rats in... 1002876 - The epidermal component of melanocytic naevi. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biological chemistry Volume: 391 ISSN: 1437-4315 ISO Abbreviation: Biol. Chem. Publication Date: 2010 Nov |
Date Detail:
|
Created Date: 2010-11-22 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
|
Languages: eng Pagination: 1333-9 Citation Subset: IM |
Affiliation:
|
Leibniz-Institut für umweltmedizinische Forschung (IUF), Auf'm Hennekamp 50, Düsseldorf, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Carbon* / toxicity Cell Communication* / drug effects Cells, Cultured Connexin 43 / metabolism Connexins / metabolism Epithelial Cells / metabolism, ultrastructure Gap Junctions* / drug effects, metabolism Isoquinolines Lung / cytology, metabolism Nanoparticles / toxicity Phosphorylation Rats Receptor, Epidermal Growth Factor / metabolism Silicon Dioxide* / toxicity beta Catenin / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Connexin 43; 0/Connexins; 0/Egfr protein, rat; 0/Isoquinolines; 0/beta Catenin; 7440-44-0/Carbon; 7631-86-9/Silicon Dioxide; 77944-88-8/lucifer yellow; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The C2-streptavidin delivery system promotes the uptake of biotinylated molecules in macrophages and...
Next Document: Detection of extracellular 8-oxo-7,8-dihydro-2'-deoxyguanosine as a biomarker of oxidative damage in...