Document Detail


Loss of gap junctional intercellular communication in rat lung epithelial cells exposed to carbon or silica-based nanoparticles.
MedLine Citation:
PMID:  20868226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to investigate whether fine and ultrafine carbon black (fC and ufC), and fine and ultrafine silica (fS, ufS) particles affect gap junctional intercellular communication (GJIC) in rat lung epithelial cells. Exposure of cells to subcytotoxic doses of ufC, fS and ufS resulted in a 63%, 59% and 77% reduction of GJIC, respectively, as determined in a dye transfer assay. In contrast to ufC, fC did not significantly alter GJIC. Changes in subcellular localization of the major gap junction protein in RLE cells, connexin-43 (Cx43), and of β-catenin were observed in cells exposed to ufC, fS or ufS. The loss of GJIC was counteracted by N-acetyl cysteine and was largely prevented by specific inhibitors of epidermal growth factor receptor-dependent signaling, pointing to the crucial role of two known major mediators of nanoparticle action, namely reactive oxygen species and membrane-receptor signaling, in particle-induced modulation of GJIC.
Authors:
Niloofar Ale-Agha; Catrin Albrecht; Lars-Oliver Klotz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological chemistry     Volume:  391     ISSN:  1437-4315     ISO Abbreviation:  Biol. Chem.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-22     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9700112     Medline TA:  Biol Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1333-9     Citation Subset:  IM    
Affiliation:
Leibniz-Institut für umweltmedizinische Forschung (IUF), Auf'm Hennekamp 50, Düsseldorf, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon* / toxicity
Cell Communication* / drug effects
Cells, Cultured
Connexin 43 / metabolism
Connexins / metabolism
Epithelial Cells / metabolism,  ultrastructure
Gap Junctions* / drug effects,  metabolism
Isoquinolines
Lung / cytology,  metabolism
Nanoparticles / toxicity
Phosphorylation
Rats
Receptor, Epidermal Growth Factor / metabolism
Silicon Dioxide* / toxicity
beta Catenin / metabolism
Chemical
Reg. No./Substance:
0/Connexin 43; 0/Connexins; 0/Egfr protein, rat; 0/Isoquinolines; 0/beta Catenin; 7440-44-0/Carbon; 7631-86-9/Silicon Dioxide; 77944-88-8/lucifer yellow; EC 2.7.10.1/Receptor, Epidermal Growth Factor

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