Document Detail

Loss of dystrophin staining in cardiomyocytes: a novel method for detection early myocardial infarction.
MedLine Citation:
PMID:  23330010     Owner:  NLM     Status:  MEDLINE    
Myocardial infarction (MI) is the most frequent diagnosis made in majority of sudden death cases subjected to clinical and medicolegal autopsies. When sudden death occurs at a very early stage of MI, traditional macroscopic examination, or histological stains cannot easily detect the myocardial changes. For this reason we propose a new method for detecting MI at an early stage. Murine model of MI was used to induce MI through permanent ligation of left anterior descending branch of left coronary artery. Five groups of C57B6/J mice were used for inducing MI, which includes 20 minutes, 30 minutes, one hour, four hours and 24 hours post MI groups. One naïve group and sham-operated groups were used as controls. There is loss of dystrophin membranous staining in cardiac myocytes occurs as early as 20 minutes post myocardial infarction. This can be used as a novel method to diagnose early myocardial infarction in post mortem cases where diagnosis is unclear. In conclusion, evaluation of immunohistochemical expression of dystrophin represents a highly sensitive method for detecting early myocardial infarction due to the loss of staining in the infarcted areas. Dystrophin immunostaining can also be used to assess myocardial architecture.
Satwat Hashmi; Suhail Al-Salam
Publication Detail:
Type:  Journal Article     Date:  2013-01-15
Journal Detail:
Title:  International journal of clinical and experimental pathology     Volume:  6     ISSN:  1936-2625     ISO Abbreviation:  Int J Clin Exp Pathol     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-18     Completed Date:  2013-07-09     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101480565     Medline TA:  Int J Clin Exp Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-57     Citation Subset:  IM    
Department of Pathology, Faculty of Medicine & Health Sciences, United Arab Emirates University ALAIN PO Box 17666, UAE.
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MeSH Terms
Autopsy / methods
Death, Sudden, Cardiac / pathology
Dermatitis, Contact
Disease Models, Animal
Dystrophin / metabolism*
Early Diagnosis
Immunohistochemistry / methods*
Mice, Inbred C57BL
Myocardial Infarction / diagnosis*,  metabolism*,  pathology
Myocytes, Cardiac / metabolism*,  pathology*
Time Factors
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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