Document Detail


Loss of cell constituents from hepatocytes on centrifugation.
MedLine Citation:
PMID:  486144     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In studies of the metabolism of isolated hepatocytes, it is often necessary to measure the concentrations of cell constituents both in cells and medium. When hepatocytes are separated in the special tubes of Hems, Lund & Krebs (1975) (Biochem. J. 150, 47--50), they lose much glucose, urea and Na+, whereas there is no loss of K+, glutamate, aspartate and adenine nucleotides. Cell water is also lost, as measured by the distribution of 3H2O. This loss is mainly due to an exchange of cell water with the aqueous solution in the stems of the tubes through which the cells pass on centrifugation. In general, substances are lost only when the intracellular concentration is equal to, or lower than, the extracellular concentration. Probably solutes are lost because they travel with the water unidirectionally out of the cell. A loss of solute does not occur when the cells are centrifuged in conical tubes with a layer of silicone oil between the cell suspension and the deproteinizing layer. The reasons for the loss occurring in the special separation tubes are discussed.
Authors:
G M Sainsbury; M Stubbs; R Hems; H A Krebs
Related Documents :
1970244 - Gamma-glutamyl-transpeptidase (ggtp) and na+k(+)-atpase activities in different subpopu...
9814984 - Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylat...
9140024 - Symmetric ph dependence of buffering power in giant fused cells from frog kidney proxim...
19393034 - Protein kinase g is involved in ammonia-induced swelling of astrocytes.
16809224 - Interstitial cells of cajal in patients with constipation due to total colonic inertia.
22933044 - Sulphamoylated estradiol analogue induces antiproliferative activity and apoptosis in b...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Biochemical journal     Volume:  180     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-11-21     Completed Date:  1979-11-21     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  685-8     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Separation / methods
Centrifugation* / methods
Female
Intracellular Fluid / analysis
Liver / analysis,  cytology*
Rats
Water / analysis
Chemical
Reg. No./Substance:
7732-18-5/Water
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of renal gluconeogenesis in rats by ochratoxin.
Next Document:  Alterations in the rate of lipogenesis in vivo in maternal liver and adipose tissue on premature wea...