Document Detail

Loss of X-chromatin and X-chromosome activation in HeLa cells.
MedLine Citation:
PMID:  294766     Owner:  NLM     Status:  MEDLINE    
Activation of second X-chromosome by the action of estradiol-17 beta was studied with HeLa cells. The incidence of X-chromatin in estrogen-treated cells decreased remarkably from 55% to 6%. Further, heavy incorporation of 3H-uridine occurred in the X-chromatin bodies of the treated cells while no such labelling was observed in controls. There was also a manifold increase in the activity of the enzyme G-6-PD in steroid-treated cells, and this increase in enzyme activity could be inhibited by addition of actinomycin D. A nonestrogenic steroid such as testosterone had no effect on these parameters. These observations do indicate activation of second X-chromosome of HeLa cells by estradiol-17 beta.
S N Ghosh; P N Shah; A P Banerji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta cytologica     Volume:  23     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:    1979 Mar-Apr
Date Detail:
Created Date:  1980-04-17     Completed Date:  1980-04-17     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  169-72     Citation Subset:  IM    
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MeSH Terms
Enzyme Activation / drug effects
Estradiol / pharmacology*
Glucosephosphate Dehydrogenase / metabolism
Hela Cells / drug effects*,  enzymology,  metabolism
Sex Chromatin / drug effects*,  metabolism
Sex Chromosomes / drug effects*
Uridine / metabolism
X Chromosome / drug effects*,  metabolism
Reg. No./Substance:
50-28-2/Estradiol; 58-96-8/Uridine; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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