| Loss of the LIM domain protein Lmo4 in the mammary gland during pregnancy impedes lobuloalveolar development. | |
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MedLine Citation:
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PMID: 15856027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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LMO4, a member of the LIM-only family of zinc-finger proteins, is overexpressed in a significant proportion of breast carcinomas and acts as a negative regulator of mammary epithelial differentiation. To delineate cell types within the developing mouse mammary gland that express Lmo4, we analysed different stages of mammopoiesis by immunohistochemistry. Lmo4 was found to be highly expressed in the proliferating cap cells of the terminal end bud and in the ductal and alveolar luminal cells of the mature mammary gland but was negligible or low in myoepithelial cells. To assess the physiological role of Lmo4 in the mammary gland, we generated conditionally targeted mice lacking Lmo4 in the mammary epithelium during pregnancy. Acute loss of Lmo4 in late pregnancy impaired lobuloalveolar development, accompanied by a two-fold reduction in the percentage of BrdU-positive cells. In contrast, germline loss of Lmo4 did not alter lobuloalveolar development arising from transplanted mammary anlagen, implying the existence of a compensatory mechanism in these knockout mice. Thus, the use of a conditional targeting strategy has revealed that Lmo4 is required for proper development of the mammary gland during pregnancy and indicated that Lmo4 acts as a positive regulator of alveolar epithelial proliferation. |
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Authors:
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Eleanor Y M Sum; Mark Shackleton; Kyungmin Hahm; Richard M Thomas; Lorraine A O'Reilly; Kay-Uwe Wagner; Geoffrey J Lindeman; Jane E Visvader |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 24 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-07-14 Completed Date: 2005-08-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: England |
Other Details:
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Languages: eng Pagination: 4820-8 Citation Subset: IM |
Affiliation:
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The Walter and Eliza Hall Institute of Medical Research and Bone Marrow Research Laboratories, 1G Royal Parade, Parkville, VIC 3050, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Proliferation Female Gene Expression Regulation, Developmental Homeodomain Proteins / genetics, metabolism* Mammary Glands, Animal / anatomy & histology, growth & development*, metabolism*, secretion Mice Milk / secretion Mutation / genetics Pregnancy Transcription Factors / deficiency, genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Homeodomain Proteins; 0/Lmo4 protein, mouse; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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