Document Detail


Loss of INCREASED SIZE EXCLUSION LIMIT (ISE)1 or ISE2 increases the formation of secondary plasmodesmata.
MedLine Citation:
PMID:  20434343     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasmodesmata (PD) transport developmentally important nucleic acids and proteins between plant cells. Primary PD form during cell division and are simple, linear channels. Secondary PD form in existing cell walls after cell division and are simple, twinned, or branched. PD function undergoes a marked reduction at the mid-torpedo stage of Arabidopsis embryogenesis. Two mutants, increased size exclusion limit (ise)1 and ise2, fail to undergo this transition, and their null mutations are embryonically lethal. We investigated the ultrastructure of PD in early-, mid-, and late-torpedo-stage embryos and in young leaves. Wild-type (WT) embryos contain twinned and branched (T/B) PD at all stages, but ise1 and ise2 embryos contain significantly higher proportions of T/B PD than WT embryos. WT T/B PD formation occurs in a stage- and tissue-specific pattern that is reversed in ise1 embryos. Silencing ISE1 in Nicotiana benthamiana leaves increases the frequency of secondary PD in existing cell walls. Silencing ISE2 increases the proportion of T/B secondary PD formed. Silenced tissues exhibit increased PD-mediated movement of green fluorescent protein tracers. Thus, silencing of ISE1 and ISE2 phenocopies ise1 and ise2 mutant embryos: when wild-type ISE1 and ISE2 functions are lost, de novo production of PD occurs, leading to increased intercellular transport.
Authors:
Tessa M Burch-Smith; Patricia C Zambryski
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-04-29
Journal Detail:
Title:  Current biology : CB     Volume:  20     ISSN:  1879-0445     ISO Abbreviation:  Curr. Biol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-09-30     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  989-93     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Ltd. All rights reserved.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/GU722291
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics,  metabolism*
Arabidopsis* / anatomy & histology,  embryology,  genetics
Arabidopsis Proteins / genetics,  metabolism*
Biological Transport
Cell Communication / physiology
Cell Wall* / metabolism,  ultrastructure
DEAD-box RNA Helicases / genetics,  metabolism*
Gene Silencing
Green Fluorescent Proteins / genetics,  metabolism
Molecular Sequence Data
Plant Leaves / cytology,  genetics,  metabolism
Plasmodesmata / metabolism*,  ultrastructure
RNA Helicases / genetics,  metabolism*
RNA Nucleotidyltransferases / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
GM45244/GM/NIGMS NIH HHS; R01 GM045244/GM/NIGMS NIH HHS; R01 GM045244-18/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DRH1 protein, Arabidopsis; 147336-22-9/Green Fluorescent Proteins; EC 2.7.7.-/INCREASED SIZE EXCLUSION LIMIT2 protein, Arabidopsis; EC 2.7.7.-/RNA Nucleotidyltransferases; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.1.-/Ise1 protein, Arabidopsis; EC 3.6.4.13/DEAD-box RNA Helicases; EC 3.6.4.13/RNA Helicases
Comments/Corrections
Comment In:
Curr Biol. 2010 Jun 8;20(11):R488-90   [PMID:  20541498 ]

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