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Loss of Heterochromatin Protein 1 Gamma Reduces the Number of Primordial Germ Cells via Impaired Cell-Cycle Progression in Mice.
MedLine Citation:
PMID:  21778144     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Signals from the extra-embryonic tissues in mice determine which proximal epiblast cells become primordial germ cells (PGCs). After their specification, approximately 40 PGCs appear at the base of the allantoic bud and migrate to the genital ridges, where they expand to about 25,000 cells by Embryonic Day 13.5 (E13.5). Heterochromatin protein 1 (HP1) family members, HP1 alpha, beta, and gamma, (CBX 5, 1, and 3, respectively), are thought to induce heterochromatin structure and to regulate gene expression by binding methylated histone H3 lysine 9. We found a dramatic loss in germ cells before meiosis in HP1gamma mutant mice that we generated previously. The reduction in PGCs in HP1gamma-/- embryos was detectable from the early bud stage (E7.25) and the number of HP1gamma-/- PGCs was gradually reduced thereafter. BrdU incorporation into PGCs was significantly reduced in E7.25 as well as E12.5 HP1gamma-/- embryos. Furthermore, a lower proportion of HP1gamma-/- PGCs than wild-type PGCs was in S phase, and a higher proportion was in G1 phase at E12.5. Moreover, the proportion of p21 (Cip1)-positive HP1gamma-/- PGCs was increased, suggesting that the G1/S phase transition was inhibited. However, no difference in fate determination, migration, apoptosis, or histone modification was detected between the PGCs of control and HP1gamma-/- embryos. Therefore, the reduction in PGCs in HP1gamma-/- embryos could be caused by impaired cell cycle in PGCs. These results suggest that HP1gamma plays an important role in keeping enough germ cells by regulating the PGC cell cycle.
Authors:
Kanae Abe; Chie Naruse; Tomoaki Kato; Takumi Nishiuchi; Mitinori Saitou; Masahide Asano
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-7-20
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  -     Publication Date:  2011 Jul 
Date Detail:
Created Date:  2011-7-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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