Document Detail

Loss of Cardioprotection With Ischemic Preconditioning in Aging Hearts: Role of Sirtuin 1?
MedLine Citation:
PMID:  22960148     Owner:  NLM     Status:  Publisher    
The effectiveness of ischemic preconditioning (IPC) to protect the heart against ischemia/reperfusion injury (IRI) declines with age. The deacetylase protein sirtuin 1 (Sirt 1) confers myriad functions including longevity and cardioprotection against IRI. As such, Sirt 1 may be a potential candidate to explain the protective effect of IPC. We aim to explore the role of Sirt 1 in the loss of the cardioprotective effect of IPC with age. Isolated hearts from young (9 weeks) and older (12-18 months) Long-Evans rats were subjected to 30 minutes of global ischemia and 60 minutes of reperfusion. Preconditioning stimuli were applied with either 2 cycles of 5-minute ischemia/reperfusion or with the potent Sirt 1 agonist resveratrol (RSV, 10 µmol/L) for 15 minutes followed by a 10-minute washout before the sustained ischemia. Both IPC and RSV significantly enhanced the functional recovery of young hearts by 168% (P < .001 vs control) and 65% (P < .01 vs control), respectively, and concomitantly reduced the infarct size by 65% and 45%, but the effect was blunted in older hearts. Administration of the selective Sirt 1 inhibitor III to young hearts did not alter the protective effect of IPC. Following ischemia/reperfusion, higher Sirt 1 deacetylase activity was detected in older hearts compared to young hearts (0.48 ± 0.13 arbitrary units [AU] vs 0.17 ± 0.03 AU, P < .01) and IPC did not alter Sirt 1 deacetylase activity. In conclusion, although Sirt 1 deacetylase activity is increased with age during ischemia/reperfusion, our data suggest that the loss of the cardioprotective effect of IPC in older animals is likely to be independent of Sirt 1.
Tasneem Adam; Stephanie Sharp; Lionel H Opie; Sandrine Lecour
Related Documents :
15337698 - Natriuretic peptide receptor-c regulates coronary blood flow and prevents myocardial is...
12935688 - Age-associated decrease in 8-hydroxy-2'-deoxyguanosine (8-ohdg) immunoreactivity in the...
9754328 - Increased formation of interstitial hydroxyl radical following myocardial ischemia: pos...
8817628 - Systemic inflammatory response and the splanchnic bed in cardiopulmonary bypass.
16865298 - Detection of coronary artery disease using real-time myocardial contrast echocardiograp...
9648898 - Evaluation of a proposed panel of cardiac markers for the diagnosis of acute myocardial...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-6
Journal Detail:
Title:  Journal of cardiovascular pharmacology and therapeutics     Volume:  -     ISSN:  1940-4034     ISO Abbreviation:  J. Cardiovasc. Pharmacol. Ther.     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9602617     Medline TA:  J Cardiovasc Pharmacol Ther     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
University of Cape Town, Cape Town, South Africa.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Incidence of early radiolucent lines after glenoid component insertion for total shoulder arthroplas...
Next Document:  Greater sensitivity to nonaccidental than metric changes in the relations between simple shapes in t...