Document Detail


Loss of Aif function causes cell death in the mouse embryo, but the temporal progression of patterning is normal.
MedLine Citation:
PMID:  16788063     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Apoptosis-inducing factor (AIF) is an evolutionarily conserved, ubiquitously expressed flavoprotein with NADH oxidase activity that is normally confined to mitochondria. In mammalian cells, AIF is released from mitochondria in response to apoptotic stimuli and translocates to the nucleus where it is thought to bind DNA and contribute to chromatinolysis and cell death in a caspase-independent manner. Here we describe the consequences of inactivating Aif in the early mouse embryo. Unexpectedly, we found that both the apoptosis-dependent process of cavitation in embryoid bodies and apoptosis associated with embryonic neural tube closure occur in the absence of AIF, indicating that Aif function is not required for apoptotic cell death in early mouse embryos. By embryonic day 9 (E9), loss of Aif function causes abnormal cell death, presumably because of reduced mitochondrial respiratory chain complex I activity. Because of this cell death, Aif null embryos fail to increase significantly in size after E9. Remarkably, patterning processes continue on an essentially normal schedule, such that E10 Aif null embryos with only approximately 1/10 the normal number of cells have the same somite number as their wild-type littermates. These observations show that pattern formation in the mouse can occur independent of embryo size and cell number.
Authors:
Doris Brown; Benjamin D Yu; Nicholas Joza; Paule Bénit; Juanito Meneses; Meri Firpo; Pierre Rustin; Josef M Penninger; Gail R Martin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-06-20
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  103     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-28     Completed Date:  2006-08-23     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9918-23     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of California-San Francisco, San Francisco, CA 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis Inducing Factor / genetics,  physiology*
Body Patterning* / genetics
Cell Count
Electron Transport Complex I / metabolism*
Embryo, Mammalian / enzymology,  ultrastructure
Embryonic Development* / genetics
Female
Genes, Lethal*
Mice
Mice, Inbred Strains
Mitochondria / enzymology
Mutation
Grant Support
ID/Acronym/Agency:
R37 HD 25331/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Apoptosis Inducing Factor; 0/Pdcd8 protein, mouse; EC 1.6.5.3/Electron Transport Complex I
Comments/Corrections

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