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Losartan prevents the development of the pro-inflammatory monocytes CD14+CD16+ in haemodialysis patients.
MedLine Citation:
PMID:  22247233     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: The principal cause of mortality in haemodialysis (HD) patients is cardiovascular disease, which is linked to chronic inflammation. Recent studies have demonstrated that angiotensin II receptor AT1 antagonists have anti-inflammatory properties. In this study, we evaluated the effect of losartan on CD14(+)CD16(+) monocytes in HD patients. In addition, we developed an in vitro model to study the mechanisms by which losartan modulates these cells.METHODS: We divided 18 HD patients into two groups, based on anti-hypertensive treatment: 9 patients were treated with losartan (losartan group) and 9 received other anti-hypertensive drugs that did not affect the renin-angiotensin axis (no-losartan group). Losartan was withdrawn in five patients from the losartan group for 2 months. Ten healthy subjects were included as controls. In vitro, we studied the differentiation of monocytes from healthy donors on stimulation with interleukin (IL)-10, IL-4 and granulocyte monocytes colony-stimulating factor with or without losartan in the culture medium.RESULTS: In patients who were taking losartan, the percentage of monocytes that expressed CD14(+)CD16(+) was lower compared with patients in the no-losartan group. The percentage of CD14(+)CD16(+) was similar in the losartan group and healthy subjects. When losartan was withdrawn from five patients in the losartan group, the percentage of CD14(+)CD16(+) monocytes increased compared with before withdrawal. In vitro, when we added losartan to the culture medium, CD14(++)CD16(-) monocytes failed to differentiate into CD14(+)CD16(+) cells.CONCLUSION: Losartan acts as an immunomodulator that prevents the development of CD14(+)CD16(+) pro-inflammatory monocytes in HD patients.
Authors:
Ana Merino; Maria Antonia Alvarez-Lara; Rafael Ramirez; Julia Carracedo; Alejandro Martin-Malo; Pedro Aljama
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-13
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  -     ISSN:  1460-2385     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)/Fundación de Investigaciones Biomédicas de Córdoba (FIBICO), Córdoba, Spain.
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