| Loop mediated isothermal amplification combined with nucleic acid lateral flow strip for diagnosis of cyprinid herpes virus-3. | |
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MedLine Citation:
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PMID: 19781627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An improved loop mediated isothermal amplification (LAMP) assay for rapid, sensitive and specific detection of cyprinid herpes virus-3 (CyHV-3), also known as koi herpes virus (KHV), was developed. The lower detection limit of the CyHV-3-LAMP assay is 10 fg DNA which equivalent to 30 copies of CyHV-3 genome. Nucleic acid lateral flow assay was used for visual detection of the LAMP products. The LAMP- nucleic acid lateral flow assay relies on DNA hybridization technology and antigen-antibody reactions in combination with LAMP. For application of this assay, the biotinylated LAMP product was hybridized with a FITC-labelled specific probe for 5 min. The resulting DNA complex could be visualised as purple band at the strip test line within 5 min of sample exposure. The nucleic acid lateral flow analysis of the LAMP product was equivalent in sensitivity but more rapid than the conventional agarose gel electrophoresis. The combination of LAMP assay with the nucleic acid lateral flow analysis can simplify the diagnosis and screening of CyHV-3 as it is simple, requires very little training, does not require specialized equipment such as a thermal cycler, the results are read visually with no need to run a gel and has a high sensitivity and specificity. |
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Authors:
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Hatem Soliman; Mansour El-Matbouli |
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Publication Detail:
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Type: Journal Article Date: 2009-09-23 |
Journal Detail:
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Title: Molecular and cellular probes Volume: 24 ISSN: 1096-1194 ISO Abbreviation: Mol. Cell. Probes Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-03 Completed Date: 2010-04-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8709751 Medline TA: Mol Cell Probes Country: England |
Other Details:
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Languages: eng Pagination: 38-43 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Fish Medicine and Livestock Management, University of Veterinary Medicine, Vienna, Austria. hatemtoughan@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line, Tumor Cyprinidae / virology* Herpesviridae / genetics, isolation & purification* Herpesviridae Infections / diagnosis, virology Humans Nucleic Acid Amplification Techniques* Polymerase Chain Reaction |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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