Document Detail


Longus, a type IV pilus of enterotoxigenic Escherichia coli, is involved in adherence to intestinal epithelial cells.
MedLine Citation:
PMID:  20348256     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enterotoxigenic Escherichia coli (ETEC) is the leading bacterial cause of diarrhea in the developing world, as well as the most common cause of traveler's diarrhea. The main hallmarks of this type of bacteria are the expression of one or more enterotoxins and fimbriae used for attachment to host intestinal cells. Longus is a pilus produced by ETEC. These bacteria grown in pleuropneumonia-like organism (PPLO) broth at 37 degrees C and in 5% CO(2) produced longus, showing that the assembly and expression of the pili depend on growth conditions and composition of the medium. To explore the role of longus in the adherence to epithelial cells, quantitative and qualitative analyses were done, and similar levels of adherence were observed, with values of 111.44 x 10(4) CFU/ml in HT-29, 101.33 x 10(4) CFU/ml in Caco-2, and 107.11 x 10(4) CFU/ml in T84 cells. In addition, the E9034A Delta lngA strain showed a significant reduction in longus adherence of 32% in HT-29, 22.28% in Caco-2, and 21.68% in T84 cells compared to the wild-type strain. In experiments performed with nonintestinal cells (HeLa and HEp-2 cells), significant differences were not observed in adherence between E9034A and derivative strains. Interestingly, the E9034A and E9034A Delta lngA(pLngA) strains were 30 to 35% more adherent in intestinal cells than in nonintestinal cells. Twitching motility experiments were performed, showing that ETEC strains E9034A and E9034A Delta lngA(pLngA) had the capacity to form spreading zones while ETEC E9034A Delta lngA does not. In addition, our data suggest that longus from ETEC participates in the colonization of human colonic cells.
Authors:
Karina Mazariego-Espinosa; Ariadnna Cruz; Maria A Ledesma; Sara A Ochoa; Juan Xicohtencatl-Cortes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-26
Journal Detail:
Title:  Journal of bacteriology     Volume:  192     ISSN:  1098-5530     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-05-12     Completed Date:  2010-05-25     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2791-800     Citation Subset:  IM    
Affiliation:
Laboratorio de Bacteriología Intestinal, Departamento de Infectología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, Delegación Cuauhtémoc, 06720 México D.F., México.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion / genetics,  physiology*
Caco-2 Cells
Cell Line, Tumor
Culture Media / pharmacology
Enterotoxigenic Escherichia coli / genetics,  metabolism,  physiology*,  ultrastructure
Enzyme-Linked Immunosorbent Assay
Epithelial Cells / microbiology*,  ultrastructure
Escherichia coli Proteins / genetics,  metabolism*
Fimbriae Proteins / genetics,  metabolism*
Flow Cytometry
Fluorescent Antibody Technique
Gene Expression Regulation, Bacterial / drug effects
HT29 Cells
HeLa Cells
Humans
Intestinal Mucosa / cytology*
Microscopy, Electron, Transmission
Chemical
Reg. No./Substance:
0/Culture Media; 0/Escherichia coli Proteins; 147680-16-8/Fimbriae Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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