Document Detail

Longitudinal study of intracellular T cell cytokine production in infants compared to adults.
MedLine Citation:
PMID:  12067304     Owner:  NLM     Status:  MEDLINE    
Intracellular cytokine production in lymphocytes obtained longitudinally from 325 healthy infants aged 2-12 months was compared with adult lymphocytes using four-colour flow cytometry. Peripheral blood samples (180 microlitres) were stimulated with phorbol 12-myristate 13-acetate, ionomycin and brefeldin A to induce production and intracellular accumulation of cytokines. The method was validated by assessing reproducibility, repeatibility, ruggedness (i.e. fresh versus day-old blood samples), precision, linearity and sensitivity. Among infants, the number and percentage of T lymphocytes (helper/inducer T cell subsets and cytotoxic/suppressor T cell subsets) producing IFN-gamma (type 1) and IL4 (type 2) increased over the first year of life but remained significantly lower than levels found in adults. In both infants and adults more CD4- T cells than CD4+ T cells were induced to make IFN-gamma. Infant Th1/Th2 ratios revealed modest Th1-skewed (predominant) profiles compared to adults, which were 5-10 times higher. Infant Tc1/Tc2 ratios revealed Tc1-skewed responses which were equal to adult ratios by age 12 months. At 12 months infant Th2 responses were closer to adult levels than were Th1 cells. Intracellular cytokine detection by flow cytometry is a rapid, sensitive, rugged and precise method to characterize immune status changes over time.
R H Buck; C T Cordle; D J Thomas; T R Winship; J P Schaller; J E Dugle
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  128     ISSN:  0009-9104     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-06-17     Completed Date:  2002-08-12     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  490-7     Citation Subset:  IM    
Abbott Laboratories, Ross Products Division, Columbus, Ohio 43219, USA.
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MeSH Terms
Brefeldin A / pharmacology
Cells, Cultured
Cytokines / biosynthesis*
Intracellular Fluid / immunology
Ionomycin / pharmacology
Longitudinal Studies
Protein Synthesis Inhibitors / pharmacology
T-Lymphocytes / cytology,  drug effects,  immunology*
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
0/Cytokines; 0/Protein Synthesis Inhibitors; 16561-29-8/Tetradecanoylphorbol Acetate; 20350-15-6/Brefeldin A; 56092-81-0/Ionomycin
Erratum In:
Clin Exp Immunol 2002 Sep;129(3):571

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