| Longitudinal-relaxation-enhanced NMR experiments for the study of nucleic acids in solution. | |
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MedLine Citation:
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PMID: 19485365 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atomic-resolution information on the structure and dynamics of nucleic acids is essential for a better understanding of the mechanistic basis of many cellular processes. NMR spectroscopy is a powerful method for studying the structure and dynamics of nucleic acids; however, solution NMR studies are currently limited to relatively small nucleic acids at high concentrations. Thus, technological and methodological improvements that increase the experimental sensitivity and spectral resolution of NMR spectroscopy are required for studies of larger nucleic acids or protein-nucleic acid complexes. Here we introduce a series of imino-proton-detected NMR experiments that yield an over 2-fold increase in sensitivity compared to conventional pulse schemes. These methods can be applied to the detection of base pair interactions, RNA-ligand titration experiments, measurement of residual dipolar (15)N-(1)H couplings, and direct measurements of conformational transitions. These NMR experiments employ longitudinal spin relaxation enhancement techniques that have proven useful in protein NMR spectroscopy. The performance of these new experiments is demonstrated for a 10 kDa TAR-TAR*(GA) RNA kissing complex and a 26 kDa tRNA. |
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Authors:
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Jonathan Farjon; Jérôme Boisbouvier; Paul Schanda; Arthur Pardi; Jean-Pierre Simorre; Bernhard Brutscher |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of the American Chemical Society Volume: 131 ISSN: 1520-5126 ISO Abbreviation: J. Am. Chem. Soc. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-17 Completed Date: 2009-08-04 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 7503056 Medline TA: J Am Chem Soc Country: United States |
Other Details:
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Languages: eng Pagination: 8571-7 Citation Subset: IM |
Affiliation:
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Institut de Biologie Structurale-Jean-Pierre Ebel, UMR5075 CNRS-CEA-UJF, 41, rue Jules Horowitz, 38027 Grenoble Cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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HIV
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genetics Hydrogen Bonding Imino Sugars / chemistry Kinetics Models, Molecular Nuclear Magnetic Resonance, Biomolecular / methods* Nucleic Acid Conformation RNA / chemistry* RNA, Transfer, Val / chemistry RNA, Viral / chemistry Solutions |
| Grant Support | |
ID/Acronym/Agency:
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AI33098/AI/NIAID NIH HHS; R01 AI033098-15/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Imino Sugars; 0/RNA, Transfer, Val; 0/RNA, Viral; 0/Solutions; 63231-63-0/RNA |
| Comments/Corrections | |
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