Document Detail


Longitudinal differentiation of chromosomes of Asellus aquaticus (Crust. Isop.) by in situ nick translation using restriction enzymes and DNase I.
MedLine Citation:
PMID:  9451951     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asellus aquaticus is an isopod crustacean whose chromosomes cannot be differentiated by G- or R-banding techniques. In this work, we have obtained a longitudinal differentiation of these chromosomes by in situ nick translation using restriction enzymes (HaeIII, DraI and BamHI) and DNase I digestions. The four nucleases, with different efficiencies, have produced similar labelling patterns. Staining with DAPI, Giemsa and chromomycin A3 reveals that the DNA of the nick-translated regions is generally more resistant to extraction from the chromosome. The results obtained on the heteromorphic sex chromosome pair observed in about a quarter of the males of a natural population allow several hypotheses to be advanced on the nature and origin of chromosome dimorphism.
Authors:
R Barzotti; F Pelliccia; A Rocchi
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  5     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-23     Completed Date:  1998-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  521-6     Citation Subset:  IM    
Affiliation:
Dip. Genetica e Biol. Mol., Università La Sapienza, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosome Banding / methods*
Crustacea / genetics*
Deoxyribonuclease I*
Deoxyribonucleases, Type II Site-Specific*
Karyotyping / methods
Male
Spermatogonia
X Chromosome
Y Chromosome
Chemical
Reg. No./Substance:
EC 3.1.21.1/Deoxyribonuclease I; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific
Comments/Corrections
Erratum In:
Chromosome Res 1998 Feb;6(2):149

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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