Document Detail


Longitudinal analysis of hepatitis C virus infection and genetic drift of the hypervariable region.
MedLine Citation:
PMID:  7545928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hepatitis C virus (HCV) infections in a cohort of chimpanzees were studied retrospectively. All animals had been inoculated intravenously with materials derived from a single-source chimpanzee plasma implicated in non-A, non-B hepatitis, prepared by extensive ultracentrifugation. Anti-HCV and HCV RNA were monitored by the confirmatory line immunoassay and by an RNA-capture polymerase chain reaction method, respectively. In a chronically infected chimpanzee, HCV RNA was detectable after 32 days and throughout the acute phase, dropped transiently below detection level, and became detectable again. In 3 other chimpanzees with acute resolving infections, HCV RNA was detected 7-11 days after inoculation and became permanently undetectable after alanine aminotransferase normalization. Various anti-HCV profiles were detected among the chimpanzees. Analysis of the hypervariable region in E2/NS1 in 7 chimpanzees suggested genome stability on transmission, revealed different mutation frequencies during chronic infection, and suggested the importance of immune selection during chronic HCV infection.
Authors:
L J van Doorn; W Quint; K Tsiquaye; J Voermans; D Paelinck; T Kos; G Maertens; H Schellekens; K Murray
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of infectious diseases     Volume:  169     ISSN:  0022-1899     ISO Abbreviation:  J. Infect. Dis.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-06-30     Completed Date:  1994-06-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413675     Medline TA:  J Infect Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1226-35     Citation Subset:  AIM; IM    
Affiliation:
Diagnostic Center SSDZ, Delft, Netherlands.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/S71394
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Cohort Studies
DNA, Viral
Female
Gene Frequency*
Genetic Variation*
Hepacivirus / genetics*
Hepatitis C / epidemiology*,  genetics
Longitudinal Studies
Male
Molecular Sequence Data
Pan troglodytes
Retrospective Studies
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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