Document Detail

Long-term storage at -80 degrees C of hematopoietic progenitor cells with 5-percent dimethyl sulfoxide as the sole cryoprotectant.
MedLine Citation:
PMID:  9920169     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Hematopoietic progenitor cells (HPCs) can be cryopreserved and stored below -120 degrees C in liquid nitrogen or at -80 degrees C in mechanical freezers. STUDY DESIGN AND METHODS: The feasibility of long-term storage of HPCs at -80 degrees C was investigated. The studies included a comparison of 5- and 10-percent dimethyl sulfoxide (DMSO) as cryoprotectant at various lengths of storage time. Mononuclear cell (MNC) recovery and viability and colony-forming unit-granulocyte-macrophage (CFU-GM) and burst-forming unit-erythroid (BFU-E) recovery assays were performed. The peripheral blood HPCs of 24 consecutive patients included in the program of autologous transplantation were studied. RESULTS: The MNC viability decreased progressively with the length of time from cryopreservation, reaching 32 percent after 31 months of storage. The recovery rates of CFU-GM and BFU-E also decreased progressively with the duration of frozen storage, to 50 and 43.5 percent, respectively, after 12 months and to 0 percent (both) after 24 months. At 6 months of storage, MNC viability was 80 percent, and CFU-GM and BFU-E recovery was 63.5 and 80.5 percent, respectively. There were no differences between MNCs cryopreserved with 5- or 10-percent DMSO in terms of cell viability. There were no differences between CFU-GM recovery or BFU-E recovery from HPCs cryopreserved in 5- or 10-percent DMSO. Patients given HPCs stored in these conditions for periods ranging between 123 and 202 days showed a complete and rapid hematologic recovery. CONCLUSION: HPCs can be cryopreserved at -80 degrees C with 5-percent DMSO and stored at -80 degrees C no longer than 6 months. A 5-percent DMSO concentration is comparable to a with 10-percent concentration in terms of recovery and MNC viability.
A Galmés; J Besalduch; J Bargay; A Novo; M Morey; J M Guerra; M A Duran
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transfusion     Volume:  39     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-02-03     Completed Date:  1999-02-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  70-3     Citation Subset:  IM    
Servicio de Hematologia y Hemoterapia, Hospital Universitario Son Dureta, Palma De Mallorca, Spain.
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MeSH Terms
Cell Survival
Child, Preschool
Cryoprotective Agents / pharmacology*
Dimethyl Sulfoxide / pharmacology*
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells*
Leukocyte Count
Leukocytes, Mononuclear / cytology
Middle Aged
Neoplasms / therapy
Neutrophils / cytology
Platelet Count / drug effects
Time Factors
Reg. No./Substance:
0/Cryoprotective Agents; 67-68-5/Dimethyl Sulfoxide

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