| Long-term proliferation and characterization of human spermatogonial stem cells obtained from obstructive and non-obstructive azoospermia under exogenous feeder-free culture conditions. | |
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MedLine Citation:
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PMID: 20590666 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: The aim of the present study was to improve efficiency of isolation and to optimize proliferative potential of human spermatogonial stem cells (SSCs) obtained from obstructive azoospermic (OA) and non-obstructive azoospermic (NOA) patients, and further, to characterize these cells for potential use in infertility treatment or study of reproductive biology. MATERIALS AND METHODS: We have applied a cell-sorting method, using collagen and magnetic activated cell separation to overcome obstacles, developing a collection system, and simple long-term proliferation system, that yields large numbers of high-purity SSCs from obstructive OA and NOA patients. RESULTS: SSCs derived from OA and NOA patients proliferated and maintained their characteristics for more than 12 passages (>6 months) in vitro. Moreover, the population of cells positive for the SSC-specific markers GFRalpha-1 and integrin alpha6, increased to more than 80% at passage 8. CONCLUSION: These finding may support the idea that in vitro propagation of SSCs could be a useful tool for infertility treatment and study of reproductive biology. |
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Authors:
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J J Lim; S-Y Sung; H J Kim; S-H Song; J Y Hong; T K Yoon; J K Kim; K-S Kim; D R Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell proliferation Volume: 43 ISSN: 1365-2184 ISO Abbreviation: Cell Prolif. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-01 Completed Date: 2010-07-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105195 Medline TA: Cell Prolif Country: England |
Other Details:
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Languages: eng Pagination: 405-17 Citation Subset: IM |
Affiliation:
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Fertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Azoospermia Cell Separation Humans Male Research Spermatogonia / cytology* Stem Cells / cytology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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