Document Detail


Long-term preservation of freeze-dried mouse spermatozoa.
MedLine Citation:
PMID:  22326411     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4°C of mouse spermatozoa freeze-dried using a simple buffer solution (10mM Tris, 1mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4°C for 3years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains.
Authors:
Takehito Kaneko; Tadao Serikawa
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-4
Journal Detail:
Title:  Cryobiology     Volume:  -     ISSN:  1090-2392     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Inc.
Affiliation:
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan.
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