| Long-term preservation of freeze-dried mouse spermatozoa. | |
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MedLine Citation:
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PMID: 22326411 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Many genetically engineered mice strains have been generated worldwide and sperm preservation is a valuable method for storing these strains as genetic resources. Freeze-drying is a useful sperm preservation method because it requires neither liquid nitrogen nor dry ice for preservation and transportation. We report here successful long-term preservation at 4°C of mouse spermatozoa freeze-dried using a simple buffer solution (10mM Tris, 1mM EDTA, pH 8.0). Offspring with fertility were obtained from oocytes fertilized with freeze-dried spermatozoa from C57BL/6 and B6D2F1 mouse strains stored at 4°C for 3years. This freeze-drying method is a safe and economical tool for the biobanking of valuable mouse strains. |
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Authors:
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Takehito Kaneko; Tadao Serikawa |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-2-4 |
Journal Detail:
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Title: Cryobiology Volume: - ISSN: 1090-2392 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-2-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0006252 Medline TA: Cryobiology Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan. |
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Descriptor/Qualifier:
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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