| Long-term perfusion culture of hybridoma: A "grow or die" cell cycle system. | |
| | |
MedLine Citation:
|
PMID: 18600804 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
In order to elucidate the hybridoma life cycle and the limiting factors in perfusion systems, we performed cultures in a stirred tank bioreactor, coupled to an external tangential flow filtration unit. Cell density and antibody production in perfusion were consistent with previous studies. The average life span of the cells (2.1-2.2 days), antibody, productivity per cell produced (30-38 mg/10(9) cells) and cell size diameter evolution appeared similar to values observed in batch cultures. These observations highly suggest a similar "grow or die" life cycle. Cell and antibody production, strictly related to the medium perfusion rate, seem to be under the control of the nutrient availability. A hypothesis to explain such a life cycle of hybridoma cells in perfusion systems and a model for viable and dead cell density is proposed. |
| | |
Authors:
|
D de la Broise; M Noiseux; R Lemieux; B Massie |
Related Documents
:
|
20554944 - Essential role of trpc6 channels in g2/m phase transition and development of human glioma. 6163954 - Progress towards a unified theory of the mechanisms of carcinogenesis: role of cell cyc... 18003834 - E2f1 works as a cell cycle suppressor in mature neurons. 10783254 - Rb function in cell-cycle regulation and apoptosis. 19816564 - Caenorhabditis elegans myotubularin mtm-1 negatively regulates the engulfment of apopto... 6355174 - Isoenzyme-specific localization of m-line bound creatine kinase in myogenic cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Biotechnology and bioengineering Volume: 38 ISSN: 0006-3592 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 1991 Oct |
Date Detail:
|
Created Date: 2008-07-08 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
|
Languages: eng Pagination: 781-7 Citation Subset: - |
Affiliation:
|
Croix Rouge Canadienne, Centre de Québec, 2535 Bld Laurier, Sainte-Foy, P. Q., Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A model of interorganelle monoclonal antibody transport and secretion in mouse hybridoma cells.
Next Document: The effect of electrochemical regeneration upon the enzymatic catalysis of a thermodynamically unfav...