Document Detail


Long-term monitoring of pulmonary arterial pressure in conscious, unrestrained mice.
MedLine Citation:
PMID:  16338149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: The ability to genetically engineer specific gene 'knock-out' mice has provided a powerful tool for investigating the various mechanisms that contribute to the pathogenesis of pulmonary arterial hypertension (PAH). Yet, so far there have been no reports describing the measurement of pulmonary arterial pressure (PAP) in the conscious wild type mouse-an essential requirement for monitoring dynamic changes associated with the pathogenesis of PAH. Therefore, in this study we describe a new technique for long-term measurement of PAP in conscious unrestrained mice using telemetry. METHODS: In five male C57BL/6 mice (B.W. 25-30 g), the sensing catheter of a telemetric transmitter was inserted into the right ventricle and advanced into the pulmonary artery. The transmitter body was positioned either within the abdominal cavity or subcutaneously on the back. During recovery from surgery, mean PAP was recorded daily for 1 week. Subsequently, the PAP responses to acute hypoxia (8% O2 for 10 min) and L-NAME (50 mg/kg, s.c.) were tested in three mice. RESULTS: By 1-week post surgery, all mice had fully recovered from surgery and baseline MPAP was stable at 14.9+/-0.7 mm Hg. Additionally, the pulmonary vascular stimulants acute hypoxia and L-NAME provoked a 63% and 86% increase MPAP, respectively. DISCUSSION: In summary, this study has demonstrated the ability to accurately measure PAP by telemetry in conscious, unrestrained mice. One important application of this technique for future studies is the possibility to assess the relative contribution of specific genes (using 'knock-out' mice) during the chronic development of pulmonary pathological conditions.
Authors:
Daryl O Schwenke; James T Pearson; Hidezo Mori; Mikiyasu Shirai
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-12-09
Journal Detail:
Title:  Journal of pharmacological and toxicological methods     Volume:  53     ISSN:  1056-8719     ISO Abbreviation:  J Pharmacol Toxicol Methods     Publication Date:    2006 May-Jun
Date Detail:
Created Date:  2006-05-15     Completed Date:  2006-08-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206091     Medline TA:  J Pharmacol Toxicol Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  277-83     Citation Subset:  IM    
Affiliation:
Department of Cardiac Physiology, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. schwenke@ri.ncvc.go.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / physiopathology
Blood Pressure / drug effects
Blood Pressure Monitoring, Ambulatory / instrumentation*,  methods*
Consciousness
Enzyme Inhibitors / pharmacology
Heart Rate / drug effects
Male
Mice
Mice, Inbred C57BL
NG-Nitroarginine Methyl Ester / administration & dosage,  pharmacology
Nitric Oxide Synthase / antagonists & inhibitors
Organ Size / drug effects
Pulmonary Artery / drug effects*,  physiology
Reference Values
Telemetry / instrumentation*,  methods*
Time Factors
Chemical
Reg. No./Substance:
0/Enzyme Inhibitors; 50903-99-6/NG-Nitroarginine Methyl Ester; EC 1.14.13.39/Nitric Oxide Synthase

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