Document Detail

Long-term inhalation of diesel exhaust affects cytokine expression in murine lung tissues: comparison between low- and high-dose diesel exhaust exposure.
MedLine Citation:
PMID:  12217215     Owner:  NLM     Status:  MEDLINE    
The authors investigated the effect of diesel exhaust (DE) on cytokine expression in murine lung tissues. BALB/c mice were exposed to DE for 1 month at different dose levels of DE (low dose: diesel exhaust particles [DEP] 100 micro g/m(3); high dose: 3mg/m(3)). After exposure, the authors examined mRNA expression of cytokines (tumor nocrosis factor alpha [TFN-alpha], Interleukin [IL]-1beta, IL-4, IL-6, IL-10, IL-12p40, and interferon gamma [IFN-gamma] and inducible nitric oxide synthase (iNOS) in the lung, and also measured the secretion of TNF-alpha and IL-10 protein by alveolar macrophages (AM). The mRNA expression levels of inflammatory cytokines (TNF-alpha, IL-1beta, IL-6, IL-12p40, IFN-gamma) and iNOS, which are important for host defense, were suppressed significantly. However, the IL-10 mRNA level was increased by DE exposure. The IL-4 mRNA level was increased by low-dose DE exposure but suppressed by high-dose DE exposure. TNF-alpha and IL-10 secretion by AM paralleled mRNA expression. Chronic inhalation of DE affects cytokine expression in murine lung. These results suggest that DE alters immunological responses in the lung and may increase susceptibility to pathogens, and that increased IL-4 expression by low-dose DE exposure may induce allergic reaction such as asthma.
Yoshinobu Saito; Arata Azuma; Shoji Kudo; Hajime Takizawa; Isamu Sugawara
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental lung research     Volume:  28     ISSN:  0190-2148     ISO Abbreviation:  Exp. Lung Res.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-09     Completed Date:  2002-10-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8004944     Medline TA:  Exp Lung Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  493-506     Citation Subset:  IM    
Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.
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MeSH Terms
Administration, Inhalation
Bronchoalveolar Lavage Fluid / cytology
Carbon Monoxide / analysis
Cytokines / genetics,  metabolism*
Dose-Response Relationship, Drug
Lung / metabolism*,  pathology
Macrophages, Alveolar / metabolism
Mice, Inbred BALB C
Nitric Oxide Synthase / genetics
Nitric Oxide Synthase Type II
Nitrites / analysis
Particle Size
RNA, Messenger / metabolism
Sulfur Dioxide / analysis
Time Factors
Vehicle Emissions* / analysis
Reg. No./Substance:
0/Cytokines; 0/Nitrites; 0/RNA, Messenger; 0/Vehicle Emissions; 630-08-0/Carbon Monoxide; 7446-09-5/Sulfur Dioxide; EC Oxide Synthase; EC Oxide Synthase Type II; EC protein, mouse

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