Document Detail


Long-term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor.
MedLine Citation:
PMID:  21309085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Peripheral lymphoid organs (PLOs), the primary sites of development of adaptive immune responses, display a complex structural organization reflecting separation of cellular subsets (e.g., T and B lymphocytes) and functional compartments which is critical for immune function. The generation of in vitro culture systems capable of recapitulating salient features of PLOs for experimental, biotechnological, and clinical applications would be highly desirable, but has been hampered so far by the complexity of these systems. We have previously developed a three-dimensional bioreactor system for long-term, functional culture of human bone marrow cells on macroporous microspheres in a packed-bed bioreactor with frequent medium change. Here we adapt the same system for culture of human primary cells from PLOs (tonsil) in the absence of specific exogenous growth factors or activators. Cells in this system displayed higher viability over several weeks, and maintain population diversity and cell surface markers largely comparable to primary cells. Light microscopy showed cells organizing in large diverse clusters within the scaffold pores and presence of B cell-enriched areas. Strikingly, these cultures generated a significant number of antibody-producing B cells when challenged with a panel of diverse antigens, as expected from a lymphoid tissue. Thus the three-dimensional tonsil bioreactor culture system may serve as a useful model of PLOs by recapitulating their structural organization and function ex vivo.
Authors:
Igor Kuzin; Hongliang Sun; Safiekhatoon Moshkani; Changyong Feng; Athanasios Mantalaris; J H David Wu; Andrea Bottaro
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-18
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  108     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-04-13     Completed Date:  2011-08-02     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1430-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
Department of Medicine, University of Rochester School of Medicine and Dentistry, URMC 695, 601 Elmwood Ave., Rochester, New York 14642, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / immunology
B-Lymphocytes / cytology,  immunology
Bioreactors*
Cell Survival
Cells, Cultured
Humans
Lymphocytes / cytology*,  immunology*
Palatine Tonsil / cytology*
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
N01 AI50029/AI/NIAID NIH HHS; N01-AI-50029/AI/NIAID NIH HHS; U19 AI-067733-01/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies
Comments/Corrections

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