| Long-term igf-I exposure decreases autophagy and cell viability. | |
| | |
MedLine Citation:
|
PMID: 20830296 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
A reduction in IGF-I signaling has been found to increase lifespan in multiple organisms despite the fact that IGF-I is a trophic factor for many cell types and has been found to have protective effects against multiple forms of damage in acute settings. The increase in longevity seen in response to reduced IGF-I signaling suggests that there may be differences between the acute and chronic impact of IGF-I signaling. We have examined the possibility that long-term stimulation with IGF-I may have a negative impact at the cellular level using quiescent human fibroblasts. We find that fibroblast cells exposed to IGF-I for 14 days have reduced long-term viability as judged by colony forming assays, which is accompanied by an accumulation of senescent cells. In addition we observe an accumulation of cells with depolarized mitochondria and a reduction in autophagy in the long-term IGF-I treated cultures. An examination of mice with reduced IGF-I levels reveals evidence of enhanced autophagy and fibroblast cells derived from these mice have a larger mitochondrial mass relative to controls indicating that changes in mitochondrial turnover occurs in animals with reduced IGF-I. The results indicate that chronic IGF-I stimulation leads to mitochondrial dysfunction and reduced cell viability. |
| | |
Authors:
|
Alessandro Bitto; Chad Lerner; Claudio Torres; Michaela Roell; Marco Malaguti; Viviana Perez; Antonello Lorenzini; Silvana Hrelia; Yuji Ikeno; Michelle Elizabeth Matzko; Roger McCarter; Christian Sell |
Related Documents
:
|
1935786 - Inhibition of human breast cancer cell (mcf-7) growth in vitro by the somatostatin anal... 8557766 - In vitro changes in plasma membrane heparan sulfate proteoglycans and in perlecan expre... 21669276 - Typha latifolia l. fruit polysaccharides induce the differentiation and stimulate the p... 22302096 - The aurora kinase a inhibitor mln8237 enhances cisplatin-induced cell death in esophage... 17355206 - Beta galactosidase complementation: a cell-based luminescent assay platform for drug di... 1935786 - Inhibition of human breast cancer cell (mcf-7) growth in vitro by the somatostatin anal... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-07 |
Journal Detail:
|
Title: PloS one Volume: 5 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-09-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
|
Languages: eng Pagination: e12592 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
AG022443/AG/NIA NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reassortant between human-Like H3N2 and avian H5 subtype influenza A viruses in pigs: a potential pu...
Next Document: Plant-type trehalose synthetic pathway in cryptosporidium and some other apicomplexans.