| Long-term high-fat diet-induced obesity decreases the cardiac leptin receptor without apparent lipotoxicity. | |
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MedLine Citation:
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PMID: 21457721 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aims Leptin resistance has been associated with cardiac lipotoxicity; however, whether leptin resistance is a risk factor associated with cardiac lipotoxicity at different time points in diet-induced obesity is unclear. The objective of this study was to evaluate this relationship. Main methods Male Wistar rats were fed a normal chow diet (12% from fat) or a high-fat diet (49% from fat) for 15 and 45weeks, respectively. The adiposity index, body weight and co-morbidities were evaluated. Heart lipotoxicity was assessed by analyzing cardiac function and morphological changes as well as cardiac triglyceride, ceramide and lipid hydroperoxide accumulations. Cardiac apoptosis was examined using the TUNEL method. Leptin function was determined by examining plasma leptin levels, cardiac leptin receptors (OB-R) and related phosphorylations of AMP-activated kinase protein (AMPK) and Acetyl CoA carboxylase (ACC). Key findings The diet-induced obesity was characterized by an elevated adiposity index, body weight and leptin levels at both 15 and 45weeks. There was no difference between groups in the cardiac triglyceride or lipid hydroperoxide levels. Interestingly, ceramide levels decreased in obese animals in both experimental periods. The cardiac morphological and functional parameters were not altered. Although down-regulation of OB-R has occurred in chronic obesity, it did not adversely affect AMPK or ACC phosphorylation. Significance The development of obesity via long-term feeding of a high-fat diet to rats does not result in cardiac lipotoxicity but promotes the down-regulation of OB-R. However, this does not result in altered levels of AMPK or ACC phosphorylations in this animal model. |
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Authors:
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André F Nascimento; Renata A M Luvizotto; André S Leopoldo; Ana P Lima-Leopoldo; Fábio R Seiva; Luís A Justulin; Meli Dal Pai Silva; Katashi Okoshi; Xiang-Dong Wang; Antonio C Cicogna |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-29 |
Journal Detail:
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Title: Life sciences Volume: - ISSN: 1879-0631 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375521 Medline TA: Life Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Medicine Clinical, Botucatu School of Medicine, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil; Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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