Document Detail

Long-term engraftment of bone marrow-derived cells in the intimal hyperplasia lesion of autologous vein grafts.
MedLine Citation:
PMID:  18276778     Owner:  NLM     Status:  MEDLINE    
Intimal hyperplasia of autologous vein grafts is a critical problem affecting the long-term patency of many types of vascular reconstruction. Within intimal hyperplasia lesions, smooth muscle cells are a major component, playing an essential role in the pathological process. Given that bone marrow-derived cells may differentiate into smooth muscle cells in the neointima of injured arteries, we hypothesized that the bone marrow may serve as a source for some of the smooth muscle cells within intimal hyperplasia lesions of vein grafts. To test this hypothesis, we used an established mouse model for intimal hyperplasia in wild-type mice that had been transplanted with bone marrow from a green fluorescent protein (GFP+/+) transgenic mouse. High-resolution confocal microscopy analysis performed 2 and 8 weeks after grafting demonstrated expression of GFP in 5.4 +/- 0.8% and 11.9 +/- 2.3%, respectively, of smooth muscle cells within intimal hyperplasia lesions. By 16 weeks, GFP expression in smooth muscle cells was not detected by immunohistochemistry; however, real-time PCR revealed that 20.2 +/- 1.7% of the smooth muscle cells captured from the neointima lesion by laser capture microdissection at 16 weeks contained GFP DNA. Our results suggest that bone marrow-derived cells differentiated into smooth muscle cells within the intimal lesion and may provide a novel clinical approach for decreasing intimal hyperplasia in vein grafts.
Yanpeng Diao; Steve Guthrie; Shen-Ling Xia; Xiaosen Ouyang; Li Zhang; Jing Xue; Pui Lee; Maria Grant; Edward Scott; Mark S Segal
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-14
Journal Detail:
Title:  The American journal of pathology     Volume:  172     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-29     Completed Date:  2008-05-09     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  839-48     Citation Subset:  AIM; IM    
Division of Nephrology, Hypertension and Transplantation, University of Florida, Gainesville, FL, USA.
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MeSH Terms
Bone Marrow Transplantation / physiology*
Cell Proliferation
Green Fluorescent Proteins / genetics,  metabolism
Hyperplasia / therapy
Mice, Inbred C57BL
Mice, Transgenic
Myocytes, Smooth Muscle / metabolism,  physiology
Time Factors
Transplantation, Autologous
Treatment Outcome
Tunica Intima / pathology*
Veins / transplantation*
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins
Comment In:
Am J Pathol. 2008 Mar;172(3):566-70   [PMID:  18276790 ]

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