Document Detail

Long-term effects of exposure to methylnitrosourea on blastocysts following transfer to surrogate female mice.
MedLine Citation:
PMID:  6722807     Owner:  NLM     Status:  MEDLINE    
The effect of in vitro preimplantation exposure of mouse embryos to N-methyl-N-nitrosourea (MNU) on development subsequent to embryo transfer into pseudopregnant surrogate mothers has been investigated. The offspring developed from mouse blastocysts exposed to MNU or to solvent were examined carefully for 1 year after birth and then examined with X-rays and complete autopsies. Metaphase chromosomes were examined in some of these animals. Exposure to MNU at the morula stage did not affect progression to the blastocyst stage in vitro; however, exposure at the blastocyst stage resulted in a profound reduction in the live birth rate. Offspring developed from blastocysts exposed to MNU in vitro were found to have a 3-fold-higher crude mortality rate than offspring developed from blastocysts exposed to solvent. There were no gross dysmorphogenic effects of exposure, nor were any major histological abnormalities associated with MNU exposure. No chromosomal abnormalities were discovered in either surviving group of offspring.
P M Iannaccone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  44     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-07-20     Completed Date:  1984-07-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2785-9     Citation Subset:  IM    
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MeSH Terms
Abnormalities, Drug-Induced / pathology
Blastocyst / drug effects*,  physiology
Embryo Transfer*
Methylnitrosourea / toxicity
Mice, Inbred BALB C
Morula / physiology
Grant Support
Reg. No./Substance:

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