Document Detail

Long-term cultures of mouse bone marrow cells: a model for studying the generation of natural killer cells.
MedLine Citation:
PMID:  1477494     Owner:  NLM     Status:  MEDLINE    
We investigated the generation of natural killer (NK) cells, using a long-term bone marrow culture (LTBMC) system. Mouse bone marrow cells were cultured for 2 weeks in complete medium without growth factors to obtain an enriched population of NK precursor cells. When these cells were recultured in the presence of interleukin-2 (IL-2) and conditioned medium (CM) from LTBMC, lytic NK cells were generated within 4 days. Replacing CM with fresh medium, before adding IL-2, decreased NK cell generation markedly, suggesting that endogenous factors present in CM were necessary for IL-2 induction of NK cells. NK cell precursors were also cultured with a combination of IL-2 and interferon-gamma (IFN-gamma) or IL-2 and tumor necrosis factor-alpha (TNF-alpha), but no CM. Results show that IFN-gamma and TNF-alpha were able to substitute CM. The addition of anti-IFN-gamma or anti-TNF-alpha antibodies to LTBMC cells, cultured in the presence of IL-2 and CM, inhibited cytotoxicity induction in a dose-dependent manner. The data indicate that IFN-gamma and TNF-alpha production may be required for IL-2 induction of NK activity, and are consistent with the hypothesis that NK generation involves collaboration between IL-2 and other bone marrow microenvironmental growth factors.
E Ayroldi; L Cannarile; F D'Adamio; D Delfino; G Migliorati; C Riccardi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Natural immunity     Volume:  11     ISSN:  1018-8916     ISO Abbreviation:  Nat. Immun.     Publication Date:    1992 Nov-Dec
Date Detail:
Created Date:  1993-02-11     Completed Date:  1993-02-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9206126     Medline TA:  Nat Immun     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  317-27     Citation Subset:  IM    
Institute of Pharmacology, School of Medicine, University of Perugia, Italy.
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MeSH Terms
Antibodies, Monoclonal
Bone Marrow / immunology*
Bone Marrow Cells
Cell Differentiation
Cells, Cultured
Culture Media
Cytotoxicity, Immunologic
Flow Cytometry
Interferon-gamma, Recombinant / immunology
Interleukin-2 / immunology
Killer Cells, Natural / cytology,  immunology*
Longitudinal Studies
Mice, Inbred C57BL
Models, Biological
Recombinant Proteins
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha / immunology
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Culture Media; 0/Interferon-gamma, Recombinant; 0/Interleukin-2; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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