Document Detail

Long-term culture of postnatal mouse hepatic stem/progenitor cells and their relative developmental hierarchy.
MedLine Citation:
PMID:  17218396     Owner:  NLM     Status:  MEDLINE    
Few studies on the long-term culture of postnatal mouse hepatic stem/progenitor cells have been reported. We successfully adapted a serum-free culture system that we employed previously to expand fetal mouse hepatic stem/progenitor cells and maintained them in culture over long periods. The expanded postnatal cells contained immature alpha-fetoprotein-positive cells along with hepatocytic and cholangiocytic lineage-committed cells. These cells expressed CD49f but not CD45, CD34, Thy-1, c-kit, CD31, or flk-1, and oncostatin M induced their differentiation. This heterogeneous population contained side population (SP) cells, which express the ATP-binding cassette transporter ABCG2, and sca-1+ cells. As mice aged, the frequency of SP and sca-1+ cells decreased along with the ability of cultured cells to expand. Approximately 20%-40% of the SP cells expressed sca-1, but only a few sca-1+ cells were also SP cells. Analysis of colonies derived from single SP or sca-1+ cells revealed that, although both cells had dual differentiation potential and self-renewal ability, SP cells formed colonies more efficiently and gave rise to SP and sca-1+ cells, whereas sca-1+ cells generated only sca-1+ progeny. Thus, SP cells are more characteristic of stem cells than are sca-1+ cells. In regenerating livers, ABCG2+ cells and sca-1+ cells were detected around or in the portal area (the putative hepatic stem cell niche). The expanded cells share many features of fetal hepatic stem/progenitor cells or oval cells and may be useful in determining the mechanisms whereby hepatic stem cells self-renew and differentiate.
Atsunori Tsuchiya; Toshio Heike; Shiro Baba; Hisanori Fujino; Katsutsugu Umeda; Yasunobu Matsuda; Minoru Nomoto; Takafumi Ichida; Yutaka Aoyagi; Tatsutoshi Nakahata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-11
Journal Detail:
Title:  Stem cells (Dayton, Ohio)     Volume:  25     ISSN:  1066-5099     ISO Abbreviation:  Stem Cells     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-10     Completed Date:  2007-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9304532     Medline TA:  Stem Cells     Country:  United States    
Other Details:
Languages:  eng     Pagination:  895-902     Citation Subset:  IM    
Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
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MeSH Terms
Aging / physiology*
Cell Culture Techniques / methods
Cell Differentiation
DNA Primers
Flow Cytometry
Gene Expression Regulation, Developmental
Liver / cytology*,  growth & development,  physiology
Mice, Inbred C57BL
RNA / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology*,  physiology*
Reg. No./Substance:
0/DNA Primers; 63231-63-0/RNA

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