Document Detail


Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney.
MedLine Citation:
PMID:  18958594     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.
Authors:
P Ciba; S Schicktanz; E Anders; E Siegl; A Stielow; E Klink; C Kruse
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-08
Journal Detail:
Title:  Fish physiology and biochemistry     Volume:  34     ISSN:  1573-5168     ISO Abbreviation:  Fish Physiol. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-29     Completed Date:  2008-12-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955049     Medline TA:  Fish Physiol Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  367-72     Citation Subset:  IM    
Affiliation:
Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Luebeck, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology
Animals
Antibodies, Monoclonal / metabolism
Cells, Cultured
Fishes / physiology*
Immunohistochemistry
Keratins / metabolism
Kidney / cytology*
Nerve Tissue Proteins / metabolism
RNA-Binding Proteins / metabolism
Time Factors
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Nerve Tissue Proteins; 0/RNA-Binding Proteins; 147605-06-9/high density lipoprotein binding protein; 68238-35-7/Keratins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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