| Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney. | |
MedLine Citation:
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PMID: 18958594 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined. |
Authors:
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P Ciba; S Schicktanz; E Anders; E Siegl; A Stielow; E Klink; C Kruse |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-08 |
Journal Detail:
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Title: Fish physiology and biochemistry Volume: 34 ISSN: 1573-5168 ISO Abbreviation: Fish Physiol. Biochem. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-10-29 Completed Date: 2008-12-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100955049 Medline TA: Fish Physiol Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 367-72 Citation Subset: IM |
Affiliation:
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Fraunhofer Institute for Marine Biotechnology, Fraunhofer Society, Luebeck, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology Animals Antibodies, Monoclonal / metabolism Cells, Cultured Fishes / physiology* Immunohistochemistry Keratins / metabolism Kidney / cytology* Nerve Tissue Proteins / metabolism RNA-Binding Proteins / metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Nerve Tissue Proteins; 0/RNA-Binding Proteins; 147605-06-9/high density lipoprotein binding protein; 68238-35-7/Keratins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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