Document Detail

Long-term cannulation model for blood sampling and intragastric infusion in the rat.
MedLine Citation:
PMID:  6433728     Owner:  NLM     Status:  MEDLINE    
An improved methodology is described for long-term venous and gastric cannulation in the rat. The long-term efficacy of the cannulas for blood sampling and intragastric infusion of liquid diet was determined in 18 rats. No animals died after surgery, and weight gain was normal. During the first 6 wk, blood could be drawn repetitively in 94% of the rats, and continuous intragastric infusion of diet was maintained in all the animals. For an extended period of 11 wk, success rates for blood sampling and dietary infusion were 75 and 100%, respectively. Standard hematological, histopathological, and clinical laboratory tests showed no abnormal changes. Furthermore, plasma corticosterone levels were consistently low (0.5 +/- 0.11 to 1.52 +/- 0.55 micrograms/dl) from the third postoperative day throughout a period of 6 wk, indicating that animals were under minimal stress.
H Tsukamoto; R D Reidelberger; S W French; C Largman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  247     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1984 Sep 
Date Detail:
Created Date:  1984-10-24     Completed Date:  1984-10-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  R595-9     Citation Subset:  IM    
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MeSH Terms
Blood Specimen Collection / methods*
Catheterization / instrumentation,  methods*,  standards
Corticosterone / blood
Enteral Nutrition / methods*
Intubation, Gastrointestinal / instrumentation,  methods*,  standards
Rats, Inbred Strains
Time Factors
Grant Support
Reg. No./Substance:

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