| Long term anoxia in rainbow trout investigated by 2-DE and MS/MS. | |
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MedLine Citation:
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PMID: 18240135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Twenty-four hours of N(2) induced anoxia induced global perturbations on protein expression in rainbow trout hypodermal fibroblasts cell line. Anoxia was obtained by depleting the medium of O(2) by flushing with N(2), and protein changes were studied by 2-DE coupled with MS providing quantitative measurements of a large number of proteins in one single study. The anoxic insult changed the level of 33 protein spots: 22 of these were up-regulated compared to the control situation and 11 were down-regulated. Using MS/MS sequencing 19 of the 33 protein spots that changed were identified, corresponding to a success rate of more than 50%. The identified proteins included two proteins involved in energy metabolism namely phosphoglycerate mutase and isocitrate dehydrogenase. In addition we observed the up-regulation of a cluster of proteins that contribute to cytoskeleton function. These are calpain, EB1, and Rho GDP dissociation inhibitor (GDI). The up-regulation of Rho GDI was shown to develop in a time dependent manner with no significant increase for up to 8 h of anoxia. In conclusion, this study provides a thorough investigation of the effect of anoxia in a cell line from rainbow trout. |
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Authors:
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Tune Wulff; Flemming Jessen; Peter Roepstorff; Else K Hoffmann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Proteomics Volume: 8 ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2008 Mar |
Date Detail:
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Created Date: 2008-03-12 Completed Date: 2008-04-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: Germany |
Other Details:
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Languages: eng Pagination: 1009-18 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Institute of Molecular Biology, University of Copenhagen, Copenhagen, Denmark. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia* Cell Line Electrophoresis, Gel, Two-Dimensional Fibroblasts / chemistry* Fish Proteins / analysis* Oncorhynchus mykiss / physiology* Proteomics* Tandem Mass Spectrometry |
| Chemical | |
Reg. No./Substance:
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0/Fish Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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