Document Detail

Long-term adaptation of breast tumor cell lines to high concentrations of nitric oxide.
MedLine Citation:
PMID:  20480412     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO), a free radical, has been implicated in the biology of human cancers, including breast cancer, yet it is still unclear how NO affects tumor development and propagation. We herein gradually adapted four human breast adenocarcinoma cell lines (BT-20, Hs578T, T-47D, and MCF-7) to increasing concentrations of the NO donor DETA-NONOate up to 600 muM. The resulting model system consisted of a set of fully adapted high nitric oxide ("HNO") cell lines that are biologically different from the "parent" cell lines from which they originated. Although each of the four parent and HNO cell lines had identical morphologic appearance, the HNO cells grew faster than their corresponding parent cells and were resistant to both nitrogen- and oxygen-based free radicals. These cell lines serve as a novel tool to study the role of NO in breast cancer progression and potentially can be used to predict the therapeutic response leading to more efficient therapeutic regimens.
Benjamin J Vesper; Kim M Elseth; Gabor Tarjan; G Kenneth Haines; James A Radosevich
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-05-18
Journal Detail:
Title:  Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine     Volume:  31     ISSN:  1423-0380     ISO Abbreviation:  Tumour Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-17     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8409922     Medline TA:  Tumour Biol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  267-75     Citation Subset:  IM    
Center for Molecular Biology of Oral Diseases, College of Dentistry, University of Illinois at Chicago, 801 S. Paulina St., Chicago, IL 60612, USA.
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MeSH Terms
Adaptation, Physiological / drug effects*
Adenocarcinoma / metabolism,  pathology*
Breast Neoplasms / metabolism,  pathology*
Cell Line, Tumor
Cell Proliferation
Cell Survival / drug effects
Hydrogen Peroxide / toxicity
Nitric Oxide / metabolism
Nitric Oxide Donors / pharmacology*
Nitroso Compounds / pharmacology*
Reg. No./Substance:
0/Nitric Oxide Donors; 0/Nitroso Compounds; 10102-43-9/Nitric Oxide; 146724-94-9/2,2'-(hydroxynitrosohydrazono)bis-ethanamine; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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