Document Detail


Long-term activation of semicarbazide-sensitive amine oxidase lowers circulating levels of uric acid in diabetic conditions.
MedLine Citation:
PMID:  22480418     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Uric acid is involved in nitrogenous waste in animals, together with ammonia and urea. Uric acid has also antioxidant properties and is a surrogate marker of metabolic syndrome. We observed that the elevated plasma uric acid of high-fat fed mice was normalized by benzylamine treatment. Indeed, benzylamine is the reference substrate of semicarbazide-sensitive amine oxidase (SSAO), an enzyme highly expressed in fat depots and vessels, which generates ammonia when catalysing oxidative deamination. Ammonia interferes with uric acid metabolism/solubility. Our aim was therefore to investigate whether the lowering action of benzylamine on uric acid was related to an improvement of diabetic complications, or was connected with SSAO-dependent ammonia production. First, we observed that benzylamine administration lowered plasma uric acid in diabetic db/db mice while it did not modify uric acid levels in normoglycemic and lean mice. In parallel, benzylamine improved the glycemic control in diabetic but not in normoglycemic mice, while plasma urea remained unaltered. Then, uric acid plasma levels were measured in mice invalidated for AOC3 gene, encoding for SSAO. These mice were unable to oxidize benzylamine but were not diabetic and exhibited unaltered plasma uric levels. Therefore, activated or abolished ammonia production by SSAO was without influence on uric acid in the context of normoglycemia. Our observations confirm that plasma uric acid increases with diabetes and can be normalized when glucose tolerance is improved. They also show that uric acid, a multifunctional metabolite at the crossroads of nitrogen waste and of antioxidant defences, can be influenced by SSAO, in a manner apparently related to changes in glucose homeostasis.
Authors:
C Carpéné; A Desquesnes; A Gomez-Ruiz; Z Iffiú-Soltész; S Le Gonidec; J Mercader
Related Documents :
22816768 - Bioavailability, distribution and anti-oxidative effects of selected triterpenes in mice.
23785538 - Reprogramming neutral lipid metabolism in mouse dendritic leucocytes hosting live leish...
14528458 - Paraffin spheres as porogen to fabricate poly(l-lactic acid) scaffolds with improved cy...
8456648 - Effects of long-term running exercise on canine femoral head articular cartilage.
11325728 - Effects of lung surfactant proteins, sp-b and sp-c, and palmitic acid on monolayer stab...
3419538 - On the epileptogenic effects of kainic acid and dihydrokainic acid in the dentate gyrus...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-5
Journal Detail:
Title:  Physiological research / Academia Scientiarum Bohemoslovaca     Volume:  -     ISSN:  1802-9973     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9112413     Medline TA:  Physiol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
INSERM U1048, équipe 3, I2MC, Bat. L4, CHU Rangueil, BP 84225, Toulouse Cedex 4, France. christian.carpene@inserm.fr.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Non invasive cardiac vein mapping: Role of multislice CT coronary angiography.
Next Document:  An antagonist of GABA-B receptors potentiates activity of cortical epileptic foci.