Document Detail


Long-term Repeated-Batch Operation of Immobilized Escherichia coli Cells to Synthesize Galactooligosaccharide.
MedLine Citation:
PMID:  23124339     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In this study, we investigated whether galactooligosaccharide (GOS) can be stably and steadily synthesized using immobilized beta-galactosidase (beta-gal) inclusion body (IB)- containing E. coli cells during long-term repeated-batch operation. To improve the operational stability of this enzyme reactor system, immobilized E. coli cells were crosslinked with glutaraldehyde (GA) after immobilization of the E. coli. When we treated with 2% GA for E. coli crosslinking, GOS production continued to an elapsed time of 576 h, in which seven batch runs were operated consecutively. GOS production ranged from 51.6 to 78.5 g/l (71.2 +/- 10.5 g/l, n = 7) during those batch operations. In contrast, when we crosslinked E. coli with 4% GA, GOS production ranged from 31.5 to 64.0 g/l (52.3 +/- 10.8, n = 4), and only four consecutive batch runs were operated. Although we did not use an industrial beta-gal for GOS production, in which a thermophile is used routinely, this represents the longest operation time for GOS production using E. coli beta-gal. Improved stability and durability of the cell immobilization system were achieved using the crosslinking protocol. This strategy could be directly applied to other microbial enzyme reactor systems using cell immobilization to extend the operation time and/or improve the reactor system stability.
Authors:
Sang-Eun Lee; Ji-Hyeon Yeon; Kyung-Hwan Jung
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microbiology and biotechnology     Volume:  22     ISSN:  1738-8872     ISO Abbreviation:  J. Microbiol. Biotechnol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9431852     Medline TA:  J Microbiol Biotechnol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  1486-93     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Korea National University of Transportation, Jeungpyung, Chungbuk 368-701, Korea.
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