Document Detail


Long lived nonadherent rabbit macrophages obtained from spleen cell cultures.
MedLine Citation:
PMID:  6826199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During the preparation of rabbit-mouse hybridomas, an unusual degree of cellular proliferation and a high level of rabbit immunoglobulin secretion in culture was noted. The proliferation seemed to be dependent on the use of relatively high levels (10%) of fetal bovine serum because it was not seen when spleen cells were cultured using either horse serum or autologous serum in a variety of media. The peak of the proliferative response occurs at 7 to 10 d in culture, after which the lymphoblast population dies rapidly, leaving a large, granular, nonadherent cell as the predominant component in the culture. These cells did not seem to divide further but persisted in very gradually diminishing numbers for many months. This long lived cell has been characterized as a nonadherent macrophage by its morphology, intense esterase staining, expression of an Fc receptor for rabbit IgG, phagocytosis of latex particles and opsonized sheep erythrocytes, and mediation of antibody dependent cell mediated cytotoxicity on chicken erythrocytes.
Authors:
J A Sogn; S Jackson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro     Volume:  19     ISSN:  0073-5655     ISO Abbreviation:  In Vitro     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-21     Completed Date:  1983-04-21     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0063733     Medline TA:  In Vitro     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  90-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody-Dependent Cell Cytotoxicity
Cell Division
Cells, Cultured
Immunoglobulins / metabolism
Macrophages / physiology*,  ultrastructure
Microscopy, Electron
Rabbits
Receptors, Fc / metabolism
Spleen / cytology*
Thymidine / metabolism
Chemical
Reg. No./Substance:
0/Immunoglobulins; 0/Receptors, Fc; 50-89-5/Thymidine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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