| Long chain saturated fatty acids increase haptoglobin gene expression in C57BL/6J mice adipose tissue and 3T3-L1 cells. | |
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MedLine Citation:
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PMID: 19856197 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Dietary lipids are directly related to the composition of adipose tissue, aetiology of obesity and arousal of obesity-related pathologies, like chronic inflammation states. Haptoglobin is an acute phase protein secreted by the liver and white adipose tissue, and its blood levels vary according to the volume of fat in the body. AIM OF THE STUDY: To investigate the effect of diets enriched with large amounts of dietary fats, which differ in their fatty acid composition, on the haptoglobin gene expression by visceral and subcutaneous adipose tissue of mice fed for 2 days or 8 weeks. 3T3-L1 cells were treated with fatty acids that are found in those types of dietary fats. METHODS: Mice were treated acutely (for 2 days) or chronically (for 8 weeks) with diets enriched with soybean oil, fish oil, coconut oil or lard. 3T3-L1 cells were treated with six different fatty acids. Haptoglobin gene expression was quantified by northern blotting. RESULTS: Both chronic and acute treatment with lard, which is rich in long chain saturated fatty acids, increased the haptoglobin mRNA expression in the retroperitoneal and epidydimal white adipose tissues. Chronic treatment with coconut oil, which is rich in medium chain saturated fatty acids, increased the haptoglobin expression in the epidydimal and subcutaneous depots. In 3T3-L1, palmitic acid increased the haptoglobin gene expression. CONCLUSION: The type of lipids in the diet can differently modulate the white adipose tissue gene expression of haptoglobin, and saturated fatty acids play a major role in promoting a pro-inflammatory environment. This response is fat pad specific and dependant on the duration of treatment. |
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Authors:
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Allain Amador Bueno; Lila Missae Oyama; Caio Sussumu de Macedo Motoyama; Carolina Rodrigues da Silva Biz; Vera Lucia Silveira; Eliane Beraldi Ribeiro; Cláudia Maria Oller do Nascimento |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-25 |
Journal Detail:
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Title: European journal of nutrition Volume: 49 ISSN: 1436-6215 ISO Abbreviation: Eur J Nutr Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-13 Completed Date: 2010-09-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100888704 Medline TA: Eur J Nutr Country: Germany |
Other Details:
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Languages: eng Pagination: 235-41 Citation Subset: IM |
Affiliation:
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Department of Physiology, São Paulo Federal University, Campus São Paulo, Rua Botucatu, 862, Edifício de Ciências Biomédicas, São Paulo, SP, Brazil. a.amadorbueno@londonmet.ac.uk |
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipocytes / metabolism Adipose Tissue, White / metabolism* Animals Blotting, Northern Diet Dose-Response Relationship, Drug Fatty Acids / pharmacology* Gene Expression / drug effects* Haptoglobins / metabolism* Lauric Acids / metabolism Mice Mice, Inbred C57BL Palmitic Acid / metabolism RNA, Messenger / metabolism Random Allocation |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/Haptoglobins; 0/Lauric Acids; 0/RNA, Messenger; 57-10-3/Palmitic Acid |
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