| Long chain-polyunsaturated fatty acids modulate membrane phospholipid composition and protein localization in lipid rafts of neural stem cell cultures. | |
| | |
MedLine Citation:
|
PMID: 20564231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Rat neural stem cells/neural progenitors (NSC/NP) are generally grown in serum-free medium. In this study, NSC/NP were supplemented with the main long-chain polyunsaturated fatty acids (PUFAs) present in the brain, arachidonic acid (AA), or docosahexaenoic acid (DHA), and were monitored for their growth. Lipid and fatty acid contents of the cells were also determined. Under standard conditions, the cells were characterized by phospholipids displaying a highly saturated profile, and very low levels of PUFAs. When cultured in the presence of PUFAs, the cells easily incorporated them into the phospholipid fraction. We also compared the presence of three membrane proteins in the lipid raft fractions: GFR and connexin 43 contents in the rafts were increased by DHA supplementation, whereas Gbeta subunit content was not significantly modified. The restoration of DHA levels in the phospholipids could profoundly affect protein localization and, consequently, their functionalities. |
| | |
Authors:
|
Bénédicte Langelier; Alain Linard; Christian Bordat; Monique Lavialle; Christine Heberden |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 110 ISSN: 1097-4644 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2010 Aug |
Date Detail:
|
Created Date: 2010-07-20 Completed Date: 2010-10-29 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 1356-64 Citation Subset: IM |
Copyright Information:
|
(c) 2010 Wiley-Liss, Inc. |
Affiliation:
|
INRA-Laboratoire de Nutrition et Régulations Lipidiques des Fonctions Cérébrales, UR909, F-78352 Jouy en Josas, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Blotting, Western Brain / cytology Cell Differentiation / drug effects Cell Proliferation / drug effects Cells, Cultured Connexin 43 / metabolism Docosahexaenoic Acids / pharmacology Epidermal Growth Factor / pharmacology Fatty Acids / analysis Fatty Acids, Unsaturated / pharmacology* Membrane Lipids / chemistry, metabolism Membrane Microdomains / drug effects*, metabolism Membrane Proteins / metabolism* Phospholipids / chemistry, metabolism* Rats Rats, Wistar Receptor, Epidermal Growth Factor / metabolism Stem Cells / cytology, drug effects*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Connexin 43; 0/Egfr protein, rat; 0/Fatty Acids; 0/Fatty Acids, Unsaturated; 0/Membrane Lipids; 0/Membrane Proteins; 0/Phospholipids; 25167-62-8/Docosahexaenoic Acids; 62229-50-9/Epidermal Growth Factor; EC 2.7.10.1/Receptor, Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Twist-ing cell fate: Mechanistic insights into the role of twist in lineage specification/differenti...
Next Document: Non-muscle myosin IIB helps mediate TNF cell death signaling independent of actomyosin contractility...