| Long-chain fatty acids differentially alter lipogenesis in bovine and caprine mammary slices. | |
| | |
MedLine Citation:
|
PMID: 23244392 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Indirect comparisons from studies in vivo have suggested that caprine mammary tissue is less sensitive than bovine mammary tissue to the anti-lipogenic effect of long-chain fatty acids (LCFA), including specific rumen biohydrogenation (RBH) intermediates of polyunsaturated fatty acids (PUFA). Our objective was to investigate the effects on lipogenesis of 18-carbon LCFA differing in the degree of unsaturation and/or double bond conformation using cultured slices of bovine and caprine mammary tissues. Mammary tissues were collected from five multiparous Holstein × Normande cows and six multiparous Alpine goats in mid lactation. The expression of genes involved in milk component synthesis was measured in tissues collected at slaughter and after slice preparation: FASN, SCD1, CD36, SREBF1 and PPARG1 mRNA levels were higher in bovine than caprine samples, whereas the opposite was observed for CSN2 mRNA levels. Bovine and caprine mammary slices were incubated for 20 h in a medium with BSA (control), cis-9-18 : 1, 18 : 2n-6, 18 : 3n-3, cis-9, trans-11-CLA, or trans-10, cis-12-CLA (the latter at 3 increasing concentrations: C1 (0·11 mm), C2 (0·16 mm), C3 (0·37 mm)). Lipogenesis was estimated by measuring the incorporation of 14C-acetate into total lipid. Significant differences of individual LCFA (P < 0·05) were observed between species: bovine tissue showed a decrease in total lipogenesis with 18 : 2n-6, 18 : 3n-3, trans-10,cis-12-CLA (C2 and C3) while caprine tissue showed an increase after treatment with 18 : 3n-3, cis-9, trans-11-CLA or trans-10, cis-12-CLA (C3). These results were not related to the mRNA abundance of our set of genes in the mammary slices after incubation. In conclusion, this study demonstrates that caprine mammary slices reacted differently from bovine mammary slices to the anti-lipogenic activity of specific LCFA and suggests that regulation of lipogenesis via other genes and/or at protein level and enzyme activity may be involved. |
| | |
Authors:
|
Laurence Bernard; Mohamad B Montazer Torbati; Benoit Graulet; Christine Leroux; Yves Chilliard |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-17 |
Journal Detail:
|
Title: The Journal of dairy research Volume: - ISSN: 1469-7629 ISO Abbreviation: J. Dairy Res. Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2012-12-18 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985125R Medline TA: J Dairy Res Country: - |
Other Details:
|
Languages: ENG Pagination: 1-7 Citation Subset: - |
Affiliation:
|
INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Low frequency repetitive transcranial magnetic stimulation to the non-lesioned hemisphere improves p...
Next Document: Conners' continuous performance test (CCPT-II) in children with ADHD, ODD, or a combined ADHD/ODD di...