Document Detail


Long-chain cationic derivatives of pta (1,3,5-triaza-7-phosphaadamantane) as new components of potential non-viral vectors.
MedLine Citation:
PMID:  22513391     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The purpose of this study was to investigate the potential of new positively charged solid lipid nanoparticles (SLN) to convey nucleic acids. The cationic character of SLN was obtained by adding as cationic molecules two different long-chain cationic phosphines (CP), namely hexadecyl-PTA iodide (CP16) and octadecyl-PTA iodide (CP18). The obtained CP-SLN are characterized by a positive charge on the surface and reproducible dimensions around 220nm. These nanosystems are able to efficiently bind nucleic acid molecules and to protect DNA from the activity of serum nucleases up to 120min. Lastly, in vitro experiments demonstrated that CP-SLN exhibit a quite pronounced antiproliferative effect on cultured human K562 erythroleukemic cells and a limited effect as transfecting adjuvant. These data, and particularly the ability of CP-SLN to protect DNA from degradation, encourages further studies aimed at proposing these nanosystems as a potential approach to deliver nucleic acid to cells in living organisms.
Authors:
Rita Cortesi; Paola Bergamini; Laura Ravani; Markus Drechsler; Andrea Costenaro; Mirko Pinotti; Matteo Campioni; Lorenza Marvelli; Elisabetta Esposito
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-15
Journal Detail:
Title:  International journal of pharmaceutics     Volume:  -     ISSN:  1873-3476     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804127     Medline TA:  Int J Pharm     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy.
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