| Long-chain acyl-CoA esters and acyl-CoA binding protein are present in the nucleus of rat liver cells. | |
| | |
MedLine Citation:
|
PMID: 10744774 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A detailed analysis of the subcellular distribution of acyl-CoA esters in rat liver revealed that significant amounts of long-chain acyl-CoA esters are present in highly purified nuclei. No contamination of microsomal or mitochondrial marker enzymes was detectable in the nuclear fraction. C16:1 and C18:3-CoA esters were the most abundant species, and thus, the composition of acyl-CoA esters in the nuclear fraction deviates notably from the overall composition of acyl-CoA esters in the cell. After intravenous administration of the non-beta-oxidizable [(14)C]tetradecylthioacetic acid (TTA), the TTA-CoA ester could be recovered from the nuclear fraction. Acyl-CoA esters bind with high affinity to the ubiquitously expressed acyl-CoA binding protein (ACBP), and several lines of evidence suggest that ACBP functions as a pool former and transporter of acyl-CoA esters in the cytoplasm. By using immunohistochemistry, immunofluorescence microscopy, and immunoelectron microscopy we demonstrate that ACBP localizes to the nucleus as well as the cytoplasm of rat liver cell and rat hepatoma cells, suggesting that ACBP may also be involved in regulation of acyl-CoA-dependent processes in the nucleus. |
| | |
Authors:
|
M Elholm; A Garras; S Neve; D Tornehave; T B Lund; J Skorve; T Flatmark; K Kristiansen; R K Berge |
Related Documents
:
|
1953734 - Effects of lymphocyte activation on transfer rnas. 8094714 - Detection of a progression-linked dna restriction fragment in rat hepatoma cells probed... 7276514 - Rex mutant in the norway rat. 1409104 - Morphological, immunohistochemical, stereological and nuclear shape characteristics of ... 959654 - The effect of cc14 on uterine ornithine decarboxylase (odc) in the rat. inhibition by c... 1335264 - Differential involvement of gustatory insular cortex and amygdala in the acquisition an... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of lipid research Volume: 41 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2000 Apr |
Date Detail:
|
Created Date: 2000-06-08 Completed Date: 2000-06-08 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 538-45 Citation Subset: IM |
Affiliation:
|
Department of Clinical Chemistry, University of Bergen, N-5021 Bergen, Norway. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acyl Coenzyme A
/
isolation & purification* Animals Antibody Specificity COS Cells Carrier Proteins / immunology, isolation & purification* Cell Compartmentation Cell Fractionation Cell Nucleus / chemistry*, ultrastructure Chromatography, High Pressure Liquid Cytoplasm / chemistry Diazepam Binding Inhibitor Fatty Acids / isolation & purification Fluorescent Antibody Technique Liver / chemistry*, ultrastructure Male Rats Rats, Sprague-Dawley Rats, Wistar Sulfides / metabolism Tissue Distribution Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/Acyl Coenzyme A; 0/Carrier Proteins; 0/Diazepam Binding Inhibitor; 0/Fatty Acids; 0/Sulfides; 2921-20-2/1-(carboxymethylthio)tetradecane |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Microsomal triglyceride transfer protein expression during mouse development.
Next Document: Phosphatidylcholine fluidity and structure affect lecithin:cholesterol acyltransferase activity.